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Identification and characterization of proteins in amniotic fluid that are differentially expressed before and after antenatal corticosteroid administration
DC Field | Value | Language |
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dc.contributor.author | Lee, JoonHo | - |
dc.contributor.author | Park, Joong Shin | - |
dc.contributor.author | Norwitz, Errol R. | - |
dc.contributor.author | Kim, Byoung Jae | - |
dc.contributor.author | Jun, Jong Kwan | - |
dc.contributor.author | Syn, Hee Chul | - |
dc.contributor.author | Park, Chan-Wook | - |
dc.date.accessioned | 2012-06-05T01:08:41Z | - |
dc.date.available | 2012-06-05T01:08:41Z | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY; Vol.202 4; - | ko_KR |
dc.identifier.issn | 0002-9378 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76825 | - |
dc.description.abstract | OBJECTIVE: We sought to examine changes in the intraamniotic proteomic environment after the administration of antenatal corticosteroids to women with impending preterm delivery. STUDY DESIGN: Amniotic fluid samples were collected at the time of clinically indicated amniocentesis before and within 7 days of administration of antenatal corticosteroids for impending preterm delivery (n = 12). Proteins differentially expressed before and after corticosteroids were identified by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. They were isolated, characterized, and quantified by fast protein liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in-gel tryptic digestion, immunodepletion assays, enzyme-linked immunosorbent assay, and electrospray ionization tandem mass spectrometry. RESULTS: Five protein peaks of interest were identified and characterized, all of which were significantly decreased after antenatal corticosteroid administration. These included 2 isoforms of transthyretin, albumin, prothrombin fragment 2, and lumican. CONCLUSION: Four proteins, identified and characterized in amniotic fluid, were differentially expressed with antenatal corticosteroid administration. These data may provide additional insight into the molecular mechanisms by which antenatal corticosteroids prevent neonatal complications. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | MOSBY-ELSEVIER | ko_KR |
dc.subject | Albumin | ko_KR |
dc.subject | transthyretin | ko_KR |
dc.subject | surface-enhanced laser desorption/ionization time-of-flight mass spectrometry | ko_KR |
dc.subject | antenatal corticosteroids | ko_KR |
dc.subject | lumican | ko_KR |
dc.subject | prothrombin fragment 2 | ko_KR |
dc.subject | amniotic fluid | ko_KR |
dc.title | Identification and characterization of proteins in amniotic fluid that are differentially expressed before and after antenatal corticosteroid administration | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이준호 | - |
dc.contributor.AlternativeAuthor | 박중신 | - |
dc.contributor.AlternativeAuthor | 김병재 | - |
dc.contributor.AlternativeAuthor | 박찬욱 | - |
dc.contributor.AlternativeAuthor | 전종관 | - |
dc.contributor.AlternativeAuthor | 신희철 | - |
dc.identifier.doi | 10.1016/j.ajog.2010.01.056 | - |
dc.citation.journaltitle | AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | - |
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dc.description.tc | 3 | - |
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