Browse

Comprehensive analysis of microRNA-mRNA co-expression in circadian rhythm

Cited 58 time in Web of Science Cited 60 time in Scopus
Authors
Na, Young-Ji; Sung, Jung Hwan; Lee, Suk Chan; Lee, Young-Ju; Park, Woong-Yang; Kim, Ju Han; Shin, Hee Sup; Choi, Yeun Joo
Issue Date
2009-09-30
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE; Vol.41 9; 638-647
Keywords
circadian rhythmgene expression profilinggene expression regulationmicroRNAmiceliver
Abstract
To investigate the potential role of microRNA (miRNA) in the regulation of circadian rhythm, we performed microarray-based expression profiling study of both miRNA and mRNA in mouse liver for 48 h at 4-hour intervals. Circadian miRNA-mRNA target pair is defined as the pair both elements of which show circadian expression patterns and the sequence-based target relationship of which can be predicted. Circadian initiators, Clock and Bmal1, showed inversely correlated circadian expression patterns against their corresponding miRNAs, miR-181d and miR-191, targeting them. In contrast, circadian suppressors, Per, Cry, Me and Rev-erba, exhibited positively correlated circadian expression patterns to their corresponding miRNAs. Genomic location analysis revealed that intronic region showed higher abundance of cyclic than non-cyclic miRNAs targeting circadian genes while other (i.e., 3`-UTR, exon and intergenic) regions showed no difference. It is suggested that miRNAs are involved in the regulation of peripheral circadian rhythm in mouse liver by modulating Clock:Bmal1 complex. Identifying specific miRNAs and their targets that are critically involved in circadian rhythm will provide a better understanding of the regulation of circadian-clock system.
ISSN
1226-3613
Language
English
URI
https://hdl.handle.net/10371/76858
DOI
https://doi.org/10.3858/emm.2009.41.9.070
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse