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A Role of Canonical Transient Receptor Potential 5 Channel in Neuronal Differentiation from A2B5 Neural Progenitor Cells

Cited 23 time in Web of Science Cited 26 time in Scopus
Authors
Shin, Hye Young; Hong, Yun Hwa; Jang, Sung Soo; Chae, Hong Gu; Moon, Hyo Eun; Kim, Jun; Kim, Sang Jeong; Paek, Sun Ha; Kim, Dong Gyu; Paek, Seung Leal
Issue Date
2010-05-07
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE; Vol.5 5; e10359
Abstract
Store-operated Ca(2+) entry (SOCE) channels are the main pathway of Ca(2+) entry in non-excitable cells such as neural progenitor cells (NPCs). However, the role of SOCE channels has not been defined in the neuronal differentiation from NPCs. Here, we show that canonical transient receptor potential channel (TRPC) as SOCE channel influences the induction of the neuronal differentiation of A2B5(+) NPCs isolated from postnatal-12-day rat cerebrums. The amplitudes of SOCE were significantly higher in neural cells differentiated from proliferating A2B5+ NPCs and applications of SOCE blockers, 2-aminoethoxy-diphenylborane (2-APB), and ruthenium red (RR), inhibited their rise of SOCE. Among TRPC subtypes (TRPC1-7), marked expression of TRPC5 and TRPC6 with turned-off TRPC1 expression was observed in neuronal cells differentiated from proliferating A2B5(+) NPCs. TRPC5 small interfering RNA (siRNA) blocked the neuronal differentiation from A2B5(+) NPCs and reduced the rise of SOCE. In contrast, TRPC6 siRNA had no significant effect on the neuronal differentiation from A2B5(+) NPCs. These results indicate that calcium regulation by TRPC5 would play a key role as a switch between proliferation and neuronal differentiation from NPCs.
ISSN
1932-6203
Language
English
URI
https://hdl.handle.net/10371/77039
DOI
https://doi.org/10.1371/journal.pone.0010359
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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