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Enhanced Reclosure of Surgically Induced Spinal Open Neural Tube Defects in Chick Embryos by Injecting Human Bone Marrow Stem Cells Into the Amniotic Cavity

Cited 10 time in Web of Science Cited 9 time in Scopus
Authors
Lee, Do-Hun; Phi, Ji Hoon; Kim, Seung-Ki; Cho, Byung-Kyu; Wang, Kyu-Chang; Kim, Seung U.
Issue Date
2010-07
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Citation
NEUROSURGERY; Vol.67 1; 129-135
Keywords
Chick embryoReclosureOpen neural tube defectHuman bone marrow stem cellHuman neural stem cell
Abstract
OBJECTIVE: To evaluate the reclosure-promoting capacity of a neural stem cell line (F3) and a human bone marrow stem cell line (B10) injected into the amniotic cavity of spinal open neural tube defects (ONTDs) of chick embryos of Hamburger and Hamilton stage 18 or 19. METHODS: Fifteen chick embryos that survived the procedure were obtained for each of 4 groups: untreated control, F3-, B10-, and HFF-1 (human foreskin fibroblast)-treated groups. Embryos in the control group underwent ONTD surgery but no cell injection. RESULTS: Compared with the untreated control and HFF-1 groups, the B10 group showed enhanced reclosure at 3, 5, and 7 days after injection, whereas the F3 group did not. B10 cells were not incorporated into reclosed neural tubes but simply covered ONTDs during the process of reclosure. F3 cells did not cover ONTDs. The cell survival of F3 cells exposed to the chick amniotic fluid in vitro for 48 hours was significantly lower than that of B10 cells. CONCLUSION: The results confirmed that B10 cells enhance reclosure of ONTDs by covering and protecting neural tissues, not by direct cell incorporation. The lack of reclosure capacity in the F3 group may be related to the poor survival of F3 cells in the amniotic fluid.
ISSN
0148-396X
Language
English
URI
https://hdl.handle.net/10371/77059
DOI
https://doi.org/10.1227/01.NEU.0000371048.76494.0F
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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