S-Space College of Medicine/School of Medicine (의과대학/대학원) Laboratory Medicine (검사의학전공) Journal Papers (저널논문_검사의학전공)
Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells
- Yoon, Jong Hyun; Roh, Eun Youn; Shin, Sue; Jung, Nam Hee; Lee, Dong Soon; Kim, Joung Sung; Jeon, Hye Won; Yoon, Kang Sup; Kim, Byoung Jae; Han, Kyou Sup; Song, Eun Young
- Issue Date
- BIOTECHNOLOGY LETTERS; Vol.31 3; 329–335
- Enzyme digestion; Growth factor; Pulsed low-intensity ultrasound; Mesenchymal stem cells; Human umbilical cord
- The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
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