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Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells

Cited 24 time in Web of Science Cited 35 time in Scopus
Authors
Yoon, Jong Hyun; Roh, Eun Youn; Shin, Sue; Jung, Nam Hee; Lee, Dong Soon; Kim, Joung Sung; Jeon, Hye Won; Yoon, Kang Sup; Kim, Byoung Jae; Han, Kyou Sup; Song, Eun Young
Issue Date
2009-03
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS; Vol.31 3; 329–335
Keywords
Enzyme digestionGrowth factorPulsed low-intensity ultrasoundMesenchymal stem cellsHuman umbilical cord
Abstract
The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
ISSN
0141-5492
Language
English
URI
http://hdl.handle.net/10371/77368
DOI
https://doi.org/10.1007/s10529-008-9872-5
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College of Medicine/School of Medicine (의과대학/대학원)Laboratory Medicine (검사의학전공)Journal Papers (저널논문_검사의학전공)
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