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Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells
Cited 27 time in
Web of Science
Cited 38 time in Scopus
- Authors
- Issue Date
- 2009-03
- Publisher
- SPRINGER
- Citation
- BIOTECHNOLOGY LETTERS; Vol.31 3; 329–335
- Keywords
- Enzyme digestion ; Growth factor ; Pulsed low-intensity ultrasound ; Mesenchymal stem cells ; Human umbilical cord
- Abstract
- The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
- ISSN
- 0141-5492
- Language
- English
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