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DIFFERENTIAL EFFECTS BETWEEN CYCLOOXYGENASE-2 INHIBITORS AND siRNA ON VASCULAR ENDOTHELIAL GROWTH FACTOR PRODUCTION IN HEAD AND NECK SQUAMOUS CELL CARCINOMA CELL LINES
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Cited 5 time in Scopus
- Authors
- Issue Date
- 2010-11
- Publisher
- JOHN WILEY & SONS INC
- Citation
- HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK; Vol.32 11; 1534-1543
- Keywords
- cyclooxygenase-2 ; small interfering RNA ; VEGF ; COX-2 ; COX-1
- Abstract
- Background. Several researchers have observed that cyclooxygenase-2 (COX-2) inhibitors display anticancer effects only at higher concentrations than doses that block COX-2 activity in head and neck squamous cell carcinoma (HNSCC) cells. Methods. To better understand the exact anticancer mechanism of COX-2-inhibitors, we compared the effects of pharmacologic inhibitors to those of small-interfering RNA against COX-2 on cell-growth, vascular endothelial growth factor (VEGF) production, and intracellular signaling in HNSCC cell lines. Results. We observed in HNSCC cells, that COX-2-siRNA induced an inhibitory effect on intracellular signaling, but unlike the pharmacologic inhibitors, did not affect cell proliferation. Whereas the chemical inhibitors increased VEGF synthesis even at low doses, COX-2-siRNA showed differential inhibition of VEGF production according to expression patterns of COX1 and COX-2 in tested cells. Conclusion. The majority of the anticancer effects of COX-2-inhibitors in HNSCC cells seem to result from COX-2-independent action, suggesting that COX-1 and COX-2 may contribute to VEGF synthesis in cancer cells through a prostaglandin-dependent mechanism. (C) 2010 Wiley Periodicals, Inc. Head Neck 32: 1534-1543,2010
- ISSN
- 1043-3074
- Language
- English
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