Browse

An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Choi, Jun Gu; Lee, Youn Jeong; Kim, Yong Joo; Lee, Eun Kyoung; Jeong, Ok Mi; Sung, Haan Woo; Kim, Jae-Hong; Kwon, Jun Hun
Issue Date
2008
Publisher
대한수의학회 = The Korean Society of Veterinary Science
Citation
J Vet Sci 2008 9, 67-74
Keywords
AIavian influenzaH9N2inactivated vaccineLPAI
Abstract
The H9N2 subtype low pathogenic avian influenza is one of the most prevalent avian diseases worldwide, and was first documented in 1996 in Korea. This disease caused serious economic loss in Korea's poultry industry. In order to develop an oil-based inactivated vaccine, a virus that had been isolated in 2001 (A/chicken/Korea/01310/ 2001) was selected based on its pathogenic, antigenic, and genetic properties. However, in animal experiments, the efficacy of the vaccine was found to be very low without concentration of the antigen (27 to 210 hemagglutinin unit). In order to overcome the low productivity, we passaged the vaccine candidate virus to chicken eggs. After the 20th passage, the virus was approximately ten times more productive compared with the parent virus. For the most part, the passaged virus maintained the hemagglutinin cleavage site amino acid motif (PATSGR/GLF) and had only three amino acid changes (T133N, V216G, E439D, H3 numbering) in the hemagglutinin molecule, as well as 18 amino acid deletions (55-72) and one amino acid change (E54D) in the NA stalk region. The amino acid changes did not significantly affect the antigenicity of the vaccine virus when tested by hemagglutination inhibition assay. Though not complete, the vaccine produced after the 20th passage of the virus (01310 CE20) showed good protection against a homologous and recent Korean isolate (A/chicken/Korea/Q30/2004) in specific pathogen- free chickens. The vaccine developed in this study would be helpful for controlling the H9N2 LPAI in Korea.
ISSN
1229-845X
Language
English
URI
http://www.vetsci.org/2008/abstract/67a.html

http://hdl.handle.net/10371/7777
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse