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In Vivo Scintigraphic Imaging of Antitumor Effects by Combined Radioiodine Therapy and Human Sodium Iodide Symporter Gene Immunotherapy

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Authors
Choi, Yun; Jeon, Yong Hyun; Paik, Jin Ho; Ko, Jinkyung; Chung, June Key; Kim, Chul Woo; Choi, Doe Han
Issue Date
2010-06
Publisher
BC DECKER INC
Citation
MOLECULAR IMAGING; Vol.9; no.3; 141-152
Abstract
In a previous study, we demonstrated that pcDNA3.1/hNIS (human sodium iodide symporter) vaccination generated hNIS-associated CD8(+)IFN-gamma(+) (interferon-gamma) T cells, which are known to be involved in antitumor immunity. However, the immune response induced was insufficient to control tumor growth in vivo, which required a novel approach to potentiate hNIS vaccination effects. In the present study, we administered (131)I radioiodine therapy prior to hNIS vaccination in CT26/hNIS tumor-bearing mice to facilitate the vaccine-induced immune response. We characterized hNIS-associated cytotoxic T-cell immune response and the antitumor effects induced by this (131)I + hNIS combination therapy. The survival rates of CT26/hNIS tumor cells were significantly reduced by (131)I treatment compared with the parental CT26 cells in vitro. (131)I + hNIS combination therapy stably suppressed tumor growth below or near the original tumor size level of initial treatment, achieving 100% survival rates. Specifically, (131)I + hNIS therapy enhanced IFN-gamma production, hNIS-associated antitumor cytotoxic T-lymphocyte (CTL) response, and induced more dendritic cells but reduced T-regulatory cells in tumor masses. Collectively, these results suggest that combined therapy effectively enhances hNIS-associated antitumor immune response, leading to CT26/hNIS tumor growth inhibition and complete survival in Balb/C mice. These findings provide a novel and effective means of treating cancer.
ISSN
1535-3508
Language
English
URI
https://hdl.handle.net/10371/77793
DOI
https://doi.org/10.2310/7290.2010.00010
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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