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A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen
DC Field | Value | Language |
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dc.contributor.author | Mun, Joohee | - |
dc.contributor.author | Kim, Yong-Hak | - |
dc.contributor.author | Yu, Jonghan | - |
dc.contributor.author | Bae, Jinhee | - |
dc.contributor.author | Yu, Myeong-Hee | - |
dc.contributor.author | Lee, Cheolju | - |
dc.contributor.author | Noh, Dong-Young | - |
dc.date.accessioned | 2012-06-29T05:29:43Z | - |
dc.date.available | 2012-06-29T05:29:43Z | - |
dc.date.issued | 2010-10 | - |
dc.identifier.citation | ELECTROPHORESIS; Vol.31(20); 3428-3436 | ko_KR |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://hdl.handle.net/10371/77940 | - |
dc.description.abstract | Autoantibodies obtained from cancer patients have been identified as useful tools for cancer diagnostics, prognostics, and as potential targets for immunotherapy. Serological proteome analysis in combination with 2-DE is a classic strategy for identification of tumor-associated antigens in the serum of cancer patients. However, serological proteome analysis cannot always indicate the true antigen out of a complex proteome identified from a single protein spot because the most abundant protein is not always the most antigenic. To address this problem, we utilized multiple parallel separation (MPS) for proteome separation. The common identities present in the fractions obtained using different separation methods were regarded as the true antigens. The merit of our MPS technique was validated using anti-ARPC2 and anti-PTEN antibodies. Next, we applied the MPS technique for the identification of glycyl-tRNA synthetase as the cognate antigen for an autoantibody that was overexpressed in the plasma of breast cancer patients. These results reveal that MPS can unambiguously identify an antibody cognate antigen by reducing false-positives. Therefore, MPS could be used for the characterization of diagnostic antibodies raised in laboratory animals as well as autoantibodies isolated from diseased patients. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | WILEY-VCH Verlag GmbH & Co. KGaA | ko_KR |
dc.subject | Autoantibody | ko_KR |
dc.subject | Multiple parallel separation | ko_KR |
dc.subject | Tumor-associated antigens | ko_KR |
dc.title | A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 문주희 | - |
dc.contributor.AlternativeAuthor | 김용학 | - |
dc.contributor.AlternativeAuthor | 유종한 | - |
dc.contributor.AlternativeAuthor | 배진희 | - |
dc.contributor.AlternativeAuthor | 노동영 | - |
dc.contributor.AlternativeAuthor | 유명희 | - |
dc.contributor.AlternativeAuthor | 이철주 | - |
dc.identifier.doi | 10.1002/elps.201000136 | - |
dc.citation.journaltitle | ELECTROPHORESIS | - |
dc.description.citedreference | Desmetz C, 2009, J PROTEOMICS, V72, P982, DOI 10.1016/j.jprot.2009.06.004 | - |
dc.description.citedreference | Achilli F, 2009, DIS MODEL MECH, V2, P359, DOI 10.1242/dmm.002527 | - |
dc.description.citedreference | Mou ZR, 2009, CANCER LETT, V278, P123, DOI 10.1016/j.canlet.2008.09.009 | - |
dc.description.citedreference | Kim HJ, 2009, J PROTEOME RES, V8, P1368, DOI 10.1021/pr8007573 | - |
dc.description.citedreference | Waller LN, 2008, J PROTEOME RES, V7, P4577, DOI 10.1021/pr8001518 | - |
dc.description.citedreference | Caron M, 2007, MOL CELL PROTEOMICS, V6, P1115, DOI 10.1074/mcp.R600016-MCP200 | - |
dc.description.citedreference | Pardo M, 2007, J PROTEOME RES, V6, P2802, DOI 10.1021/pr070021t | - |
dc.description.citedreference | Canelle L, 2006, ELECTROPHORESIS, V27, P1609, DOI 10.1002/elps.200500712 | - |
dc.description.citedreference | Gires O, 2004, CELL MOL LIFE SCI, V61, P1198, DOI 10.1007/s00018-004-4045-8 | - |
dc.description.citedreference | Imafuku Y, 2004, DIS MARKERS, V20, P149 | - |
dc.description.citedreference | Stulik J, 2003, PROTEOMICS, V3, P951, DOI 10.1002/pmic.200300370 | - |
dc.description.citedreference | Lichtenfels R, 2003, BBA-PROTEINS PROTEOM, V1646, P21, DOI 10.1016/S1570-9639(02)00547-2 | - |
dc.description.citedreference | Wasenius VM, 2003, CLIN CANCER RES, V9, P68 | - |
dc.description.citedreference | Ros A, 2002, PROTEOMICS, V2, P151 | - |
dc.description.citedreference | Brichory FM, 2001, P NATL ACAD SCI USA, V98, P9824 | - |
dc.description.citedreference | Scandurro AB, 2001, INT J ONCOL, V19, P129 | - |
dc.description.citedreference | Klade CS, 2001, PROTEOMICS, V1, P890 | - |
dc.description.citedreference | Chong BE, 2001, RAPID COMMUN MASS SP, V15, P291 | - |
dc.description.citedreference | Soussi T, 2000, CANCER RES, V60, P1777 | - |
dc.description.citedreference | Ward RL, 1999, HUM IMMUNOL, V60, P510 | - |
dc.description.citedreference | Leslie RDG, 1999, DIABETOLOGIA, V42, P3 | - |
dc.description.citedreference | Figeys D, 1998, ELECTROPHORESIS, V19, P1811 | - |
dc.description.citedreference | Yamamoto A, 1997, INTERNAL MED, V36, P724 | - |
dc.description.citedreference | Hirakata M, 1996, ARTHRITIS RHEUM, V39, P146 | - |
dc.description.citedreference | SAHIN U, 1995, P NATL ACAD SCI USA, V92, P11810 | - |
dc.description.citedreference | GE Q, 1994, J BIOL CHEM, V269, P28790 | - |
dc.description.tc | 2 | - |
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