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Protein Expression Profiling of Primary Mammary Epithelial Cells Derived from MMTV-neu Mice Revealed that HER2/NEU-Driven Changes in Protein Expression Are Functionally Clustered

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dc.contributor.authorPark, Sungwoo-
dc.contributor.authorLee, Kyung-min-
dc.contributor.authorJu, Ji-hyun-
dc.contributor.authorKim, Jaeyoon-
dc.contributor.authorLee, Taehoon-
dc.contributor.authorShin, Incheol-
dc.contributor.authorNoh, Dong-Young-
dc.identifier.citationIUBMB LIFE; Vol.62 1; 41-50ko_KR
dc.description.abstractMMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest (TM)/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis. (C) 2009 IUBMB IUBMB Life, 62(1): 41-50, 2010ko_KR
dc.description.sponsorshipThis work is supported by FPR08A2-080 of 21st Century Frontier
Functional Proteomics Project from Korean Ministry of Science
and Technology and by a grant of the Korea Healthcare
technology R&D Project, Ministry of Welfare and Family
Affairs, Republic of Korea, (A084466).
dc.publisherJOHN WILEY & SONS INCko_KR
dc.subjectsignal transductionko_KR
dc.titleProtein Expression Profiling of Primary Mammary Epithelial Cells Derived from MMTV-neu Mice Revealed that HER2/NEU-Driven Changes in Protein Expression Are Functionally Clusteredko_KR
dc.citation.journaltitleIUBMB LIFE-
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