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Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury

Cited 18 time in Web of Science Cited 0 time in Scopus
Authors
Cho, Kyung-Ah; Joo, Sun-Young; Han, Ho-Seong; Ryu, Kyung-Ha; Woo, So-Youn
Issue Date
2010-10
Publisher
SPANDIDOS PUBL LTD
Citation
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE; Vol.26; no.4; 557-563
Keywords
osteoclastsand osteoblastsSDF-1hematopoietic stem cellsCXCR4
Abstract
Osteoclasts (OCLs) are multinucleated cells that are derived from the monocyte/macrophage hematopoietic lineage in response to receptor activator of NF-kappa B ligand (RANKL) activation. They are specialized cells responsible for physiological bone resorption and as well as pathologic bone loss. In addition to their unique ability to resorb bone, OCLs also play a potential role in the mobilization of hematopoietic progenitor cells from the bone marrow (BM), particularly under various stress stimuli (e.g. hypoxia, injury or inflammation). We investigated the effect of activated OCLs on the stem cell niche and whether this leads to mobiliz-ation of hematopoietic progenitors. We induced activated OCLs from the RAW264.7 cell line through stimulation with RANKL and we quantified the levels of the stem cell niche component SDF-1 on the osteblasts and CXCR4 on the bone marrow cells (BMCs) by culturing with supernatants from activated OCLs. In addition, we exposed mice to stress by inducing liver injury with CCl(4) followed by injecting RANKL to activate OCLs and compared the effect on the mobilization of hematopoietic progenitor cells from the BM. We found that functional OCLs cleaved SDF-1 alpha in the osteoblasts and increased CXCR4 expression in the BMCs. Moreover, under stress in vivo, mobilized hematopoietic progenitor cells were significantly increased after RANKL treatment. These results suggest that OCLs might be involved in alteration of the interaction between SDF-1 and CXCR4 leading to mobilization of hematopoietic progenitor cells from the BM.
ISSN
1107-3756
Language
English
URI
https://hdl.handle.net/10371/77949
DOI
https://doi.org/10.3892/ijmm_00000499
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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