Browse

AMYLOID-PEPTIDE ACCELERATES ALTERATIONS OF EXPRESSION AND LOCALIZATION OF TIGHT JUNCTION PROTEINS VIA RAGE

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Kook, S. Y.; Son, S. M.; Hong, H.; Mook-Jung, I
Issue Date
2009-09
Publisher
WILEY-BLACKWELL PUBLISHING, INC
Citation
JOURNAL OF NEUROCHEMISTRY; Vol.110 ; 60-60
Abstract
The blood-brain barrier (BBB) is the specialized barrier formed by
the endothelial cells and an important role in regulating transport of
various molecules and maintaining integrity and brain homeostasis.
Deposition of amyloid b-peptide (Ab) in the brain is the prominent
feature of Alzheimer’s disease (AD). Accumulation of Ab leads to
increase activated microglia and astrocytes, resulting in alteration of
brain microenvironment and BBB breakdown. The brain endothelium
forming the BBB is connected by tight junction proteins
including ZO-1 and occludin, restricting paracellular permeability in
selective. The receptor for advanced glycation end products
(RAGE) binds soluble Ab and is thought to be a major transporter
of Ab across BBB. Influx of Ab by RAGE from blood to brain
elevates deposition of Ab in the brain including around BBB. From
the point of view the overexpression of RAGE in AD brain
vasculature, as well as RAGE-dependent binding and transport of
Ab, we hypothesized that enhanced RAGE-mediated Ab may
accelerate BBB disruption by inducing alteration of tight junction
proteins and paracellular permeability. We attempt to explore tight junction proteins may be altered by Ab following up-regulation of
RAGE in the immortalized mouse brain capillary endothelial cell
line, bEnd3 cells, as an in vitro BBB model. We observed that Ab
altered the expression of ZO-1 and occludin mRNA and protein as
well as the localization of those proteins in vitro. It indicates that Ab
causes the alteration of mRNA, protein and localization of tight
junction proteins and it is accelerated in RAGE-overexpressing
bEnd3 cells, suggesting that RAGE-Ab interaction might be
important for change of the BBB integrity in AD patients.
ISSN
0022-3042
Language
English
URI
http://hdl.handle.net/10371/78190
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse