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Enhancement of DC vaccine potency by activating the PI3K/AKT pathway with a small interfering RNA targeting PTEN
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Hee | - |
dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Noh, Kyung Hee | - |
dc.contributor.author | Kim, Seok-Ho | - |
dc.contributor.author | Kim, Keon Woo | - |
dc.contributor.author | Ahn, Ye-Hyeon | - |
dc.contributor.author | Kim, Jin-Seok | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.contributor.author | Choi, Eun Young | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Lee, Young-Ho | - |
dc.date.accessioned | 2012-07-03T04:08:09Z | - |
dc.date.available | 2012-07-03T04:08:09Z | - |
dc.date.issued | 2010-11-30 | - |
dc.identifier.citation | IMMUNOLOGY LETTERS; Vol.134 1; 47-54 | ko_KR |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78203 | - |
dc.description.abstract | Dendritic cell (DC)-based cancer vaccines have become Important as an immunotherapeutics in generating anti-tumor immune responses Due to a short lifespan of DCs however clinical application of current DC vaccines has been limited Recently activation of AKT/protein kinase B (PKB) a major effector of phosphatidylinositol 3-kinase (PI3K) has been reported as a critical factor in both activation and survival of DCs We here improved the potency of a DC vaccine with a small interfering RNA (siRNA) targeting phosphatase and tensin homologue (PTEN) which is known to be a central negative regulator of the PI3K/AKT signal transduction cascade Down-regulation of PTEN in DCs resulted in AKT dependent maturation which in turn caused a significant up-regulation of surface expression in co-stimulatory molecules and the chemokine receptor CCR7 leading to an increase of in vitro T cell activation activity and in vivo migration to a draining lymph node respectively Moreover these PTEN siRNA-transfected DCs (DC/siPTEN) acquired an Increased survival from the apoptotic death caused by GM-CSF deprivation or antigen-specific CD8(+) T cell killing Most importantly DC/siPTEN generated more tumor antigen-specific CD8(+) T cells and stronger anti-tumor effects in vaccinated mice than did control DCs (DC/siGFP) Thus our data indicate that manipulation of the PI3K/AKT pathway via siRNA system could improve the efficacy of a DC-based tumor vaccine (C) 2010 Elsevier B V All rights reserved | ko_KR |
dc.description.sponsorship | This work was supported by a grant from the Innovative
Research Institute for Cell Therapy, Republic of Korea (A062260), a grant from the Basic Research Program of the Korea Science & Engineering Foundation (No. R1-2006-000-10565-0), a grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare (070355), and a grant R11-2005-017-03003-0 from the Research Center for Womens Diseases of KOSEF. K.M. Lee was supported by a grant from KICOS (K20704000007-09A0500-00710). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER SCIENCE BV | ko_KR |
dc.subject | Dendritic cell | ko_KR |
dc.subject | siRNA | ko_KR |
dc.subject | Immunotherapy | ko_KR |
dc.subject | AKT | ko_KR |
dc.subject | PTEN | ko_KR |
dc.subject | PI3K | ko_KR |
dc.title | Enhancement of DC vaccine potency by activating the PI3K/AKT pathway with a small interfering RNA targeting PTEN | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김진희 | - |
dc.contributor.AlternativeAuthor | 강태흥 | - |
dc.contributor.AlternativeAuthor | 노경희 | - |
dc.contributor.AlternativeAuthor | 김석호 | - |
dc.contributor.AlternativeAuthor | 이영호 | - |
dc.contributor.AlternativeAuthor | 김건우 | - |
dc.contributor.AlternativeAuthor | 배현철 | - |
dc.contributor.AlternativeAuthor | 안예현 | - |
dc.contributor.AlternativeAuthor | 최은영 | - |
dc.contributor.AlternativeAuthor | 김진석 | - |
dc.contributor.AlternativeAuthor | 이경미 | - |
dc.contributor.AlternativeAuthor | 김태우 | - |
dc.identifier.doi | 10.1016/j.imlet.2010.08.008 | - |
dc.citation.journaltitle | IMMUNOLOGY LETTERS | - |
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