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NTA center dot Ni2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells

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Issue Date
2010-06-20
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY; Vol.31 6; 1474-1478
Keywords
Quantum dot conjugatesImaging probesProtein trackingLabeling
Abstract
An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag (His(6)-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) (NTA center dot Ni2+) on the QD surfaces. We showed that the NTA center dot Ni2+-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted His(6)-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.
ISSN
0253-2964
Language
English
URI
https://hdl.handle.net/10371/78218
DOI
https://doi.org/10.5012/bkcs.2010.31.6.1474
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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