Browse

Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination

DC Field Value Language
dc.contributor.authorPark, Kyoung-Chan-
dc.contributor.authorKim, So-Young-
dc.contributor.authorKim, Dong-Seok-
dc.date.accessioned2012-07-03T06:47:33Z-
dc.date.available2012-07-03T06:47:33Z-
dc.date.issued2009-09-
dc.identifier.citationBIOLOGICAL & PHARMACEUTICAL BULLETIN; Vol.32, no.9; 1609-1613ko_KR
dc.identifier.issn0918-6158-
dc.identifier.urihttps://hdl.handle.net/10371/78274-
dc.description.abstractRecently, indole-3-acetic acid (IAA) has been introduced as a new cancer therapeutic agent through oxidative decarboxylation by horseradish peroxidase (HRP). The purpose of this study was to determine the therapeutic feasibility of IAA/light combination against liver cancer. SK-HEP-1 cells were irradiated with UVB or visible light (518 nm) in the presence of IAA. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then, IAA was injected in SK-HEP-1 liver cancer cell-implanted nude mice, and the tumor area was irradiated with intense pulsed light (IPL). Then, tissue was taken for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay and immunohistochemical staining for 8-hydroxy-deoxyguanosine (8-OHdG), p53, caspase-3, and proliferating cell nuclear antigen (PCNA). lit vitro experiments demonstrated that IAA alone was not cytotoxic, but activated IAA by HRP or light caused cell death. lit vivo experiments showed that IAA/IPL treatment caused regression of tumor cells in SK-HEP-1-implanted nude mice. The TUNEL assay showed that IAA/IPL induced cancer cell apoptosis, and this was confirmed by increases in 8-OHdG, p53, and caspase-3 in IAA/IPL-treated mice. In contrast, IPL alone did not induce apoptosis, indicating that the apoptotic effect resulted from activated IAA by light. In summary, we showed that IAA/light induced tumor regression in SK-HEP-1-implanted nude mice. These results suggest the potential use of IAA/light combination in liver cancer.ko_KR
dc.language.isoenko_KR
dc.publisherPHARMACEUTICAL SOC JAPANko_KR
dc.subjectindole-3-acetic acidko_KR
dc.subjectintense pulsed lightko_KR
dc.subjecthepatomako_KR
dc.subjectapoptosisko_KR
dc.subjecthorseradish peroxidaseko_KR
dc.titleExperimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combinationko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor박경찬-
dc.contributor.AlternativeAuthor김소영-
dc.contributor.AlternativeAuthor김동석-
dc.identifier.doi10.1248/bpb.32.1609-
dc.citation.journaltitleBIOLOGICAL & PHARMACEUTICAL BULLETIN-
dc.description.citedreferenceKim DS, 2006, BIOL PHARM BULL, V29, P2404-
dc.description.citedreferenceKim DS, 2006, FEBS LETT, V580, P1439, DOI 10.1016/j.febslet.2006.01.073-
dc.description.citedreferenceTafazoli S, 2004, CHEM RES TOXICOL, V17, P1350-
dc.description.citedreferenceKim DS, 2004, CELL SIGNAL, V16, P81, DOI 10.1016/S0898-6568(03)00091-3-
dc.description.citedreferenceFolkes LK, 2003, CANCER RES, V63, P776-
dc.description.citedreferenceRaulin C, 2003, LASER SURG MED, V32, P78, DOI 10.1002/lsm.10145-
dc.description.citedreferenceMcCune LM, 2002, J ETHNOPHARMACOL, V82, P197, DOI 10.1016/S0378-8741(02)00180-0-
dc.description.citedreferenceWardman P, 2002, CURR PHARM DESIGN, V8, P1363-
dc.description.citedreferenceKawano T, 2001, BIOCHEM BIOPH RES CO, V288, P546-
dc.description.citedreferenceFolkes LK, 2001, BIOCHEM PHARMACOL, V61, P129, DOI 10.1016/S0006-2952(00)00498-6-
dc.description.citedreferenceFolkes LK, 1999, BIOCHEM PHARMACOL, V57, P375-
dc.description.citedreferenceFolkes LK, 1998, INT J RADIAT ONCOL, V42, P917-
dc.description.citedreferencede Melo MP, 1998, GEN PHARMACOL, V31, P573-
dc.description.citedreferenceDougherty TJ, 1998, J NATL CANCER I, V90, P889-
dc.description.citedreferenceValkonen M, 1997, J LIPID RES, V38, P823-
dc.description.citedreferenceGazaryan IG, 1996, BIOCHEM J, V313, P841-
dc.description.citedreferenceCANDEIAS LP, 1995, FREE RADICAL RES, V23, P403-
dc.description.citedreferenceGOLDSMITH MHM, 1993, P NATL ACAD SCI USA, V90, P11442-
dc.description.citedreferenceMARTINEZ E, 1983, CLIN CHEM, V29, P1354-
dc.description.citedreferenceRYSANEK K, 1959, EXPERIENTIA, V15, P217-
dc.description.citedreferenceMIRSKY IA, 1956, P SOC EXP BIOL MED, V93, P109-
dc.description.tc4-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse