Publications
Detailed Information
Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Kyoung-Chan | - |
dc.contributor.author | Kim, So-Young | - |
dc.contributor.author | Kim, Dong-Seok | - |
dc.date.accessioned | 2012-07-03T06:47:33Z | - |
dc.date.available | 2012-07-03T06:47:33Z | - |
dc.date.issued | 2009-09 | - |
dc.identifier.citation | BIOLOGICAL & PHARMACEUTICAL BULLETIN; Vol.32, no.9; 1609-1613 | ko_KR |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78274 | - |
dc.description.abstract | Recently, indole-3-acetic acid (IAA) has been introduced as a new cancer therapeutic agent through oxidative decarboxylation by horseradish peroxidase (HRP). The purpose of this study was to determine the therapeutic feasibility of IAA/light combination against liver cancer. SK-HEP-1 cells were irradiated with UVB or visible light (518 nm) in the presence of IAA. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then, IAA was injected in SK-HEP-1 liver cancer cell-implanted nude mice, and the tumor area was irradiated with intense pulsed light (IPL). Then, tissue was taken for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay and immunohistochemical staining for 8-hydroxy-deoxyguanosine (8-OHdG), p53, caspase-3, and proliferating cell nuclear antigen (PCNA). lit vitro experiments demonstrated that IAA alone was not cytotoxic, but activated IAA by HRP or light caused cell death. lit vivo experiments showed that IAA/IPL treatment caused regression of tumor cells in SK-HEP-1-implanted nude mice. The TUNEL assay showed that IAA/IPL induced cancer cell apoptosis, and this was confirmed by increases in 8-OHdG, p53, and caspase-3 in IAA/IPL-treated mice. In contrast, IPL alone did not induce apoptosis, indicating that the apoptotic effect resulted from activated IAA by light. In summary, we showed that IAA/light induced tumor regression in SK-HEP-1-implanted nude mice. These results suggest the potential use of IAA/light combination in liver cancer. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | PHARMACEUTICAL SOC JAPAN | ko_KR |
dc.subject | indole-3-acetic acid | ko_KR |
dc.subject | intense pulsed light | ko_KR |
dc.subject | hepatoma | ko_KR |
dc.subject | apoptosis | ko_KR |
dc.subject | horseradish peroxidase | ko_KR |
dc.title | Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박경찬 | - |
dc.contributor.AlternativeAuthor | 김소영 | - |
dc.contributor.AlternativeAuthor | 김동석 | - |
dc.identifier.doi | 10.1248/bpb.32.1609 | - |
dc.citation.journaltitle | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.description.citedreference | Kim DS, 2006, BIOL PHARM BULL, V29, P2404 | - |
dc.description.citedreference | Kim DS, 2006, FEBS LETT, V580, P1439, DOI 10.1016/j.febslet.2006.01.073 | - |
dc.description.citedreference | Tafazoli S, 2004, CHEM RES TOXICOL, V17, P1350 | - |
dc.description.citedreference | Kim DS, 2004, CELL SIGNAL, V16, P81, DOI 10.1016/S0898-6568(03)00091-3 | - |
dc.description.citedreference | Folkes LK, 2003, CANCER RES, V63, P776 | - |
dc.description.citedreference | Raulin C, 2003, LASER SURG MED, V32, P78, DOI 10.1002/lsm.10145 | - |
dc.description.citedreference | McCune LM, 2002, J ETHNOPHARMACOL, V82, P197, DOI 10.1016/S0378-8741(02)00180-0 | - |
dc.description.citedreference | Wardman P, 2002, CURR PHARM DESIGN, V8, P1363 | - |
dc.description.citedreference | Kawano T, 2001, BIOCHEM BIOPH RES CO, V288, P546 | - |
dc.description.citedreference | Folkes LK, 2001, BIOCHEM PHARMACOL, V61, P129, DOI 10.1016/S0006-2952(00)00498-6 | - |
dc.description.citedreference | Folkes LK, 1999, BIOCHEM PHARMACOL, V57, P375 | - |
dc.description.citedreference | Folkes LK, 1998, INT J RADIAT ONCOL, V42, P917 | - |
dc.description.citedreference | de Melo MP, 1998, GEN PHARMACOL, V31, P573 | - |
dc.description.citedreference | Dougherty TJ, 1998, J NATL CANCER I, V90, P889 | - |
dc.description.citedreference | Valkonen M, 1997, J LIPID RES, V38, P823 | - |
dc.description.citedreference | Gazaryan IG, 1996, BIOCHEM J, V313, P841 | - |
dc.description.citedreference | CANDEIAS LP, 1995, FREE RADICAL RES, V23, P403 | - |
dc.description.citedreference | GOLDSMITH MHM, 1993, P NATL ACAD SCI USA, V90, P11442 | - |
dc.description.citedreference | MARTINEZ E, 1983, CLIN CHEM, V29, P1354 | - |
dc.description.citedreference | RYSANEK K, 1959, EXPERIENTIA, V15, P217 | - |
dc.description.citedreference | MIRSKY IA, 1956, P SOC EXP BIOL MED, V93, P109 | - |
dc.description.tc | 4 | - |
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.