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Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2 `-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyue Yim | - |
dc.contributor.author | Kang, Hyungu | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Lee, Dong Soo | - |
dc.contributor.author | Kim, Soonhag | - |
dc.contributor.author | Lee, Jung Hwan | - |
dc.date.accessioned | 2012-07-03T07:43:05Z | - |
dc.date.available | 2012-07-03T07:43:05Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY; Vol. ; - | ko_KR |
dc.identifier.issn | 1110-7243 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78294 | - |
dc.description.abstract | Chemically modified nucleotides have been developed and applied into SELEX procedure to find a novel type of aptamers to fit with targets of interest. In this study, we directly performed chemical modification of 5-(N-benzylcarboxyamide)-2`-deoxyuridine (called 5-BzdU) in the AS1411 aptamer, which binds to the nucleolin protein expressed in cancer cells. Forty-seven compounds of AS1411-containing Cy3-labeled 5-BzdU (called Cy3-(5-BzdU)-modified-AS1411) were synthesized by randomly substituting thymidines one to twelve in AS1411 with Cy3-labeled 5-BzdU. Both statistically quantified fluorescence measurements and confocal imaging analysis demonstrated at least three potential compounds of interest: number 12, 29 and 41 that significantly increased the targeting affinity to cancer cells but no significant activity from normal healthy cells. These results suggest that the position and number of substituents in AS1411 are critical parameters to improve the aptamer function. In this study, we demonstrated that chemical modification of the existing aptamers enhanced the binding and targeting affinity to targets of interest without additional SELEX procedures. | ko_KR |
dc.description.sponsorship | This
work was supported by National R&D Program for Cancer Control ofMinistry of Health &Welfare (0820320), National Research Foundation of Korea (no. 20090084640), POSCO Strategy R&D program (40003503.01), and a grant of the Korea Healthcare technology R&D Project, Ministry for Health,Welfare & Family Affairs, South Korea (A085136). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | HINDAWI PUBLISHING CORPORATION | ko_KR |
dc.title | Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2 `-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이규임 | - |
dc.contributor.AlternativeAuthor | 강현구 | - |
dc.contributor.AlternativeAuthor | 류성호 | - |
dc.contributor.AlternativeAuthor | 이동수 | - |
dc.contributor.AlternativeAuthor | 이정환 | - |
dc.contributor.AlternativeAuthor | 김순학 | - |
dc.identifier.doi | 10.1155/2010/168306 | - |
dc.citation.journaltitle | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | - |
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dc.description.tc | 3 | - |
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