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UV decreases the synthesis of free fatty acids and triglycerides in the epidermis of human skin in vivo, contributing to development of skin photoaging

Cited 35 time in Web of Science Cited 39 time in Scopus
Authors
Kim, Eun Ju; Jin, Xing-Ji; Kim, Yeon Kyung; Oh, In Kyung; Park, Chi-Hyun; Chung, Jin Ho; Kim, Ji Eun
Issue Date
2010-01
Publisher
ELSEVIER IRELAND LTD
Citation
JOURNAL OF DERMATOLOGICAL SCIENCE; Vol.57 1; 19-26
Keywords
Ultraviolet radiationFree fatty acidsSterol regulatory element binding proteinsMatrix metalloproteinase-1Triglycerides
Abstract
Background: Although fatty acids are known to be important in various skin functions, their roles on photoaging in human skin are poorly understood. Objective: We investigated the alteration of lipid metabolism in the epidermis by photoaging and acute UV irradiation in human skin. Methods: UV irradiated young volunteers (21-33 years, n = 6) and elderly volunteers (70-75 years, n = 7) skin samples were obtained by punch biopsy. Then the epidermis was separated from dermis and lipid metabolism was investigated. Results: We observed that the amounts of free fatty acids (FFA) and triglycerides (TG) in the epidermis of photoaged or acutely UV irradiated human skin were significantly decreased. The expressions of genes related to lipid synthesis, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD), sterol regulatory element binding proteins (SREBPs), and peroxisome proliferator-activated receptors (PPAR gamma) were also markedly decreased. To elucidate the significance of these changes of epidermal lipids in human skin, we investigated the effects of TG or various inhibitors for the enzymes involved in TG synthesis on the expression of matrix metalloproteinase-1 (MMP-1) in cultured human epidermal keratinocytes. We demonstrated that triolein (TG) reduced basal and UV-induced MMP-1 mRNA expression. In addition, each inhibitor for various lipid synthesis enzymes, such as TOFA (ACC inhibitor), cerulenin (FAS inhibitor) and trans-10, cis-12-CLA (SCD inhibitor), increased the MMP-1 expression significantly in a dose-dependent manner. We also demonstrated that triolein could inhibit cerulenin-induced MMP-1 expression. Furthermore, topical application of triolein (10%) significantly prevented UV-induced MMP-13, COX-2, and IL-1 beta expression in hairless mice. Conclusion: Our results suggest that TG and FFA may play important roles in photoaging of human skin. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
ISSN
0923-1811
Language
English
URI
https://hdl.handle.net/10371/78356
DOI
https://doi.org/10.1016/j.jdermsci.2009.10.008
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College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
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