S-Space College of Medicine/School of Medicine (의과대학/대학원) Radiology (영상의학전공) Journal Papers (저널논문_영상의학전공)
Smart Magnetic Fluorescent Nanoparticle Imaging Probes to Monitor MicroRNAs
- Hwang, Do Won; Song, In Chan; Lee, Dong Soo; Kim, Soonhag
- Issue Date
- WILEY-V C H VERLAG GMBH
- SMALL; Vol.6 1; 81-88
- An imaging system that can be used to evaluate the expression levels of microRNAs during neuronal development can provide noninvasive information for investigating a variety of biological phenomena related to microRNAs (miRNAs, miRs). Herein, the development of a novel imaging platform to monitor intracellular miR124a during neuronal differentiation is reported using rhodamine-coated cobalt ferrite magnetic fluorescent (MF) nanoparticles linked to a quenching molecular system containing an miR124a binding sequence (MF-miR124a beacon). During neuronal differentiation, in vitro fluorescence signals of the MF-miR124a beacon are significantly increased under conditions where miR124a is highly expressed, and dramatically return to the original quenched fluorescence after anti-miR124a treatment. In vivo fluorescence images show enhanced fluorescence signals in mice with P19 cells within a poly-L-lactic acid scaffold after induction of neuronal differentiation. In addition, magnetic resonance (MR) images provide in vivo tracking of cells containing the MF-miR124a beacon. These studies represent the first step toward the use of nanotechnological imaging of mature miRNA, and this technique could be used for cellular tracking with a MR imaging system as well as for simultaneous monitoring of the miRNA expression pattern in vivo.
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