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SLA typing using the PCR-SSP method and establishment of the SLA homozygote line in pedigreed SNU miniature pigs

Cited 12 time in Web of Science Cited 12 time in Scopus
Authors

Yeom, Su-Cheong; Park, Chung-Gyu; Lee, Byeong-Chun; Lee, Wang-Jae

Issue Date
2010-04
Publisher
WILEY-BLACKWELL PUBLISHING, INC
Citation
ANIMAL SCIENCE JOURNAL; Vol.81(2); 158-164
Keywords
PCR-SSPSLA homozygoteSwine leukocyte antigen
Abstract
Seoul National University (SNU) miniature pigs represent a closed colony with 24 founder pigs and a well preserved pedigree. Characterization using mRNA sequence analysis was conducted for 6 swine leukocyte antigen (SLA) loci in parental or founder pigs, and 17 defined alleles were detected. Based on these complete coding sequences, 17 sequence specific primers (SSPs) were designed for polymorphic sites. To validate the specificity of each allele SSP, the PCR-SSP was conducted with defined allele clones as templates. PCR-SSP was conducted with the hot start polymerase and touch-down PCR. The parental or found SNU miniature pigs showed overall SLA class I and II heterozygotes. Using the established PCR-SSP method, we conducted SLA typing for breeding stock including 2 pedigreed pigs and identified the novel SLA class II homozygote haplotye (DRA*0201, DRB1*0403, DQA*0102 and DQB1*0701) and 2 SLA homozygote pig lines: SLA class I Hp-3.0 and class II Hp-0.3, and SLA class I Hp-2.0 and class II Hp-0.2. We thought that our PCR-SSP SLA typing method could be applicable for new SLA homozygote line establishment by assignment and scheduled breeding.
ISSN
1344-3941
Language
English
URI
https://hdl.handle.net/10371/78686
DOI
https://doi.org/10.1111/j.1740-0929.2009.00727.x
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