Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins

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Lee, Youn-Jeong; Sung, Haan-Woo; Choi, Jun-Gu; Lee, Eun-Kyoung; Yoon, Hachung; Kim, Jae-Hong; Song, Chang-Seon

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대한수의학회 = The Korean Society of Veterinary Science
J Vet Sci 2008, 9, 301-308
Newcastle diseaserecombinant proteinsubunit vaccine
Recombinant baculoviruses containing the fusion (F) and
hemagglutinin-neuraminidase (HN) glycoprotein gene of
the viscerotropic velogenic (vv) Newcastle disease virus
(NDV) isolate, Kr-005/00, and a lentogenic La Sota strain
of the NDV were constructed in an attempt to develop an
effective subunit vaccine to the recent epizootic vvNDV.
The level of protection was determined by evaluating the
clinical signs, mortality, and virus shedding from the
oropharynx and cloaca of chickens after a challenge with
vvNDV Kr-005/00. The recombinant ND F (rND F) and
recombinant HN (rND HN) glycoproteins derived from
the velogenic strain provided good protection against the
clinical signs and mortality, showing a 0.00 PI value and
100% protection after a booster immunization. On the
other hand, the combined rND F + HN glycoprotein
derived from the velogenic strain induced complete
protection (0.00 PI value and 100% protection) and
significantly reduced the amount of virus shedding even
after a single immunization. The rND F and rND HN
glycoproteins derived from the velogenic strain had a
slightly, but not significantly, greater protective effect
than the lentogenic strain. These results suggest that the
combined rND F + HN glycoprotein derived from vvNDV
can be an ideal subunit marker vaccine candidate in
chickens in a future ND eradication program.
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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