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Indole-3-Carbinol Prevents H2O 2-Induced Inhibition of Gap Junctional Intercellular Communication by Inactivation of PKB/Akt

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Hwang, Jae-Woong; Jung, Ji-Won; Lee, Yong Soon; Kang, Kyung-Sun
Issue Date
2008
Publisher
Japanese Society of Veterinary Science
Citation
J. Vet. Med. Sci. 70: 1057-1063
Keywords
gap junctional intercellular communicationindole-3-carbinoloxidative stressPKB/Akt
Abstract
Indole-3-carbinol (I3C) is a phytochemical found in cruciferous vegetables and possesses a variety of biological and biochemical
effects. Despite a wealth of data about the chemopreventive properties of I3C, its effects on gap junctional intercellular communication
(GJIC), which is associated with the promotion and progression phases of the multi-stage process of carcinogenesis, has not been
studied. In this study, we examined the ability of I3C to prevent H2O2-induced inhibition of GJIC in WB-F344 rat liver epithelial cells
(WB cells). The cells were preincubated with I3C for 48 hr, and then treated with 1 mM H2O2 for 1 hr. We found that I3C could prevent
the H2O2-induced inhibition of GJIC through prevention of the phosphorylated state of gap junction protein connexin 43 (Cx43) phosphorylation.
Prevention of GJIC by I3C was dependent upon inactivation of Akt, but not MAPK, although inhibition of GJIC by H2O2
leads to activation of both. Similar to I3C, modulation of Akt activation through the phosphoinositide-3 kinase inhibitor, LY294002,
could also prevent H2O2-induced inhibition of GJIC and phosphorylation of Cx43. Our results suggest that I3C might exert its dietary
chemopreventive effects by interfering with the Akt signaling pathway, which appears to be linked to modulating GJIC, a cellular mechanisms
regulating cell proliferation, differentiation and apoptosis.
ISSN
0916-7250 (print)
1347-7439 (online)
Language
English
URI
http://hdl.handle.net/10371/7905
DOI
https://doi.org/10.1292/jvms.70.1057
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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