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Patterned cell culture inside microfluidic devices
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rhee, Seog Woo | - |
dc.contributor.author | Taylor, Anne M. | - |
dc.contributor.author | Tu, Christina H. | - |
dc.contributor.author | Cribbs, David H. | - |
dc.contributor.author | Cotman, Carl W. | - |
dc.contributor.author | Jeon, Noo Li | - |
dc.date.accessioned | 2009-08-28T04:53:31Z | - |
dc.date.available | 2009-08-28T04:53:31Z | - |
dc.date.issued | 2004-07-26 | - |
dc.identifier.citation | Lab Chip, 2004, 5, 102-107 | en |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7982 | - |
dc.description.abstract | This paper describes a simple plasma-based dry etching method that enables patterned cell culture
inside microfluidic devices by allowing patterning, fluidic bonding and sterilization steps to be carried out in a single step. This plasma-based dry etching method was used to pattern celladhesive and non-adhesive areas on the glass and polystyrene substrates. The patterned substrate was used for selective attachment and growth of human umbilical vein endothelial cells, MDAMB- 231 human breast cancer cells, NIH 3T3 mouse fibroblasts, and primary rat cortical neurons. Finally, we have successfully combined the dry-patterned substrate with a microfluidic device. Patterned primary rat neurons were maintained for up to 6 days inside the microfluidic devices and the neurons somas and processes were confined to the cell-adhesive region. The method developed in this work offers a convenient way of micropatterning biomaterials for selective attachment of cells on the substrates, and enables culturing of patterned cells inside microfluidic devices for a number of biological research applications where cells need to be exposed to wellcontrolled fluidic microenvironment. | en |
dc.description.sponsorship | The Institute for Brain Aging and Dementia thanks NIA (AG17765) | en |
dc.language.iso | en | en |
dc.publisher | Royal Society of Chemistry | en |
dc.subject | pattern | en |
dc.subject | microfluidic device | en |
dc.title | Patterned cell culture inside microfluidic devices | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 전누리 | - |
dc.contributor.AlternativeAuthor | 이석우 | - |
dc.identifier.doi | 10.1039/b403091e | - |
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