Publications

Detailed Information

A Biologically Active Sequence of the Laminin alpha 2 Large Globular 1 Domain Promotes Cell Adhesion through Syndecan-1 by Inducing Phosphorylation and Membrane Localization of Protein Kinase C delta

DC Field Value Language
dc.contributor.authorJung, Sung Youn-
dc.contributor.authorKim, Jin-Man-
dc.contributor.authorJang, Da Hyun-
dc.contributor.authorKang, Hyun Ki-
dc.contributor.authorMin, Byung-Moo-
dc.date.accessioned2013-01-14T06:02:52Z-
dc.date.available2013-01-14T06:02:52Z-
dc.date.issued2009-11-13-
dc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.284, No.46, pp.31764-31775ko_KR
dc.identifier.issn0021-9258-
dc.identifier.urihttps://hdl.handle.net/10371/80384-
dc.description.abstractLaminin-2 promotes basement membrane assembly and peripheral myelinogenesis; however, a receptor-binding motif within laminin-2 and the downstream signaling pathways for motif-mediated cell adhesion have not been fully established. The human laminin-2 alpha 2 chain cDNAs cloned from human keratinocytes and fibroblasts correspond to the laminin alpha 2 chain variant sequence from the human brain. Individually expressed recombinant large globular (LG) 1 protein promotes cell adhesion and has heparin binding activities. Studies with synthetic peptides delineate the DLTIDDSYWYRI motif (Ln2-P3) within the LG1 as a major site for both heparin and cell binding. Cell adhesion to LG1 and Ln2-P3 is inhibited by treatment of heparitinase I and chondroitinase ABC. Syndecan-1 from PC12 cells binds to LG1 and Ln2-P3 and colocalizes with both molecules. Suppression of syndecan-1 with RNA interference inhibits cell adhesion to LG1 and Ln2-P3. The binding of syndecan-1 with LG1 and Ln2-P3 induces the recruitment of protein kinase C delta (PKC delta) into the membrane and stimulates its tyrosine phosphorylation. A decrease in PKC delta activity significantly reduces cell adhesion to LG1 and Ln2-P3. Taken together, these results indicate that the Ln2-P3 motif and LG1 domain, containing the motif, within the human laminin-2 alpha 2 chain are major ligands for syndecan-1, which mediates cell adhesion through the PKC delta signaling pathway.ko_KR
dc.description.sponsorshipThis work was supported by grants from the Korea Science and Engineering Foundation through the Intellectual Biointerface Engineering Center at Seoul National University (to B.-M. M.).-
dc.language.isoenko_KR
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCko_KR
dc.titleA Biologically Active Sequence of the Laminin alpha 2 Large Globular 1 Domain Promotes Cell Adhesion through Syndecan-1 by Inducing Phosphorylation and Membrane Localization of Protein Kinase C deltako_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor정성윤-
dc.contributor.AlternativeAuthor김진만-
dc.contributor.AlternativeAuthor장다현-
dc.contributor.AlternativeAuthor강현기-
dc.contributor.AlternativeAuthor민병무-
dc.identifier.doi10.1074/jbc.M109.038547-
dc.citation.journaltitleJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.description.tc3-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share