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CXCR-4 knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ji-Hong | - |
dc.contributor.author | Lee, Jae-Il | - |
dc.contributor.author | Hong, Sam-Pyo | - |
dc.contributor.author | Hong, Seong-Doo | - |
dc.contributor.author | Kim, Mi-Ae | - |
dc.contributor.author | Pai, Hyun-Kyung | - |
dc.contributor.author | Hong, Kyoung-Ok | - |
dc.contributor.author | Hong, Ji-Soo | - |
dc.date.accessioned | 2013-01-14T06:08:06Z | - |
dc.date.available | 2013-01-14T06:08:06Z | - |
dc.date.issued | 2009-02 | - |
dc.identifier.citation | JOURNAL OF ORAL PATHOLOGY & MEDICINE, Vol.38, No.2, pp.214-219 | ko_KR |
dc.identifier.issn | 0904-2512 | - |
dc.identifier.uri | https://hdl.handle.net/10371/80392 | - |
dc.description.abstract | Oral squamous cell carcinomas (OSCCs) are characterized by a high degree of local invasion and a high rate of metastases to cervical lymph nodes. Downregulation of CXCR-4 by siRNA inhibits invasion and growth of breast and colon cancer cells. However, there have been no reports on the downregulation of CXCR-4 by small interfering RNA (siRNA) in oral cancer cells. We generated two stable CXCR-4-knockdown clones (KBsi and KOSCC-25Bsi) from the KB and KOSCC-25B OSCC cell lines by lentiviral delivery. In vitro invasion and cell proliferation assays were used to investigate the effect of CXCR-4 downregulation on cell proliferation and invasiveness in KBsi and KOSCC-25Bsi. Immunohistochemistry was performed to evaluate the correlation between CXCR-4 expression and proliferation in 26 OSCC tissue samples. CXCR4-knockdown OSCC cells showed reduced invasiveness. The invasiveness of KBsi decreased to 29.5% of the vector-infected controls, and KOSCC-25Bsi decreased to 38.1% of the control vector-infected cells (P < 0.05). The CXCR4-knockdown OSCC cells grew significantly slower than the vector-infected control cells. KBsi and KOSCC-25Bsi cells proliferated at 69.5% and 71.7%, respectively, of the rate of control vector-infected cells (P < 0.05). CXCR-4-positive group had significantly higher PCNA labeling index than CXCR-4-negative group in OSCC tissue samples. These results suggest that the downregulation of CXCR-4 induces anti-proliferative and anti-invasive effects in OSCC and that CXCR-4 might be a useful target molecule for the treatment of OSCC. | ko_KR |
dc.description.sponsorship | This work was supported by grant no. 4-2005-0005 from the Seoul National University Dental Hospital Research Fund. | - |
dc.language.iso | en | ko_KR |
dc.publisher | WILEY-BLACKWELL PUBLISHING, INC | ko_KR |
dc.subject | CXCR-4 knockdown | ko_KR |
dc.subject | invasion | ko_KR |
dc.subject | proliferation | ko_KR |
dc.subject | siRNA | ko_KR |
dc.subject | oral squamous cell carcinoma | ko_KR |
dc.title | CXCR-4 knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김지홍 | - |
dc.contributor.AlternativeAuthor | 이재일 | - |
dc.contributor.AlternativeAuthor | 홍삼표 | - |
dc.contributor.AlternativeAuthor | 홍성두 | - |
dc.contributor.AlternativeAuthor | 김미애 | - |
dc.contributor.AlternativeAuthor | 배현경 | - |
dc.contributor.AlternativeAuthor | 홍경옥 | - |
dc.contributor.AlternativeAuthor | 홍지수 | - |
dc.identifier.doi | 10.1111/j.1600-0714.2008.00671.x | - |
dc.citation.journaltitle | JOURNAL OF ORAL PATHOLOGY & MEDICINE | - |
dc.description.tc | 6 | - |
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