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Comparative study of LHX8 expression between odontoma and dental tissue-derived stem cells

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dc.contributor.authorKim, Ji-Youn-
dc.contributor.authorJeon, Soung Hoo-
dc.contributor.authorPark, Joo-Young-
dc.contributor.authorSuh, Je-Duck-
dc.contributor.authorChoung, Pill-Hoon-
dc.date.accessioned2013-01-14T06:23:59Z-
dc.date.available2013-01-14T06:23:59Z-
dc.date.issued2011-03-
dc.identifier.citationJOURNAL OF ORAL PATHOLOGY & MEDICINE; Vol.40, No.3, pp.250-256ko_KR
dc.identifier.issn0904-2512-
dc.identifier.urihttps://hdl.handle.net/10371/80409-
dc.description.abstractBackground: LHX8 (LIM-homeobox gene 8) is known as an important regulating factor in tooth morphogenesis. Odontoma is a mixed odontogenic tumor where epithelium and mesenchyme differentiated together, resulting in anomalous tooth structures. In this study, gene and protein expressions of LHX8 were analyzed in human odontoma-derived mesenchymal cells (HODC) compared to adult dental mesenchymal stem cells (aDSC), as well as morphological and histological characteristics of odontoma were analyzed. Methods: aDSCs were isolated from normal teeth, and HODCs were isolated from surgically removed odontoma mass. Morphological and histological evaluations were performed to compare between compound odontomas and normal premolars. RT-PCR and real-time PCR were performed to identify LHX8 mRNA expression in the HODCs and aDSCs. LHX8 protein expression levels were observed by immunoblotting and immunofluorescent staining. Results: The compound odontoma was composed of multiple tooth-like structures, which contained disorganized but recognizable enamel matrix, dentin, pulp, and cementum. LHX8 mRNA and LHX8 protein expressions were all higher in HODCs compared to those in aDSCs examined by RT-PCR, immunoblot, and immunofluorescent staining. Especially, real-time PCR showed 2.77-fold higher LHX8 expression in HODCs than in normal periodontal ligament stem cells (PDLSCs), while alveolar bone marrow stem cells (ABMSCs) expressed 0.12-fold LHX8 than PDLSCs. Conclusions: Based on these observations, LHX8 might play an important role in odontoma formation. This is the first report regarding the comparison of LHX8 expression between HODC and normal aDSCs and its overexpression in human samples. The specific mechanism of LHX8 in odontoma morphogenesis awaits further study.ko_KR
dc.language.isoenko_KR
dc.publisherWILEY-BLACKWELLko_KR
dc.subjectdental mesenchymal stem cellko_KR
dc.subjectLHX8ko_KR
dc.subjectodontomako_KR
dc.titleComparative study of LHX8 expression between odontoma and dental tissue-derived stem cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김지연-
dc.contributor.AlternativeAuthor전성후-
dc.contributor.AlternativeAuthor박주영-
dc.contributor.AlternativeAuthor서제덕-
dc.contributor.AlternativeAuthor정필훈-
dc.identifier.doi10.1111/j.1600-0714.2010.00970.x-
dc.citation.journaltitleJOURNAL OF ORAL PATHOLOGY & MEDICINE-
dc.description.tc1-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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