Chlorpromazine과 Bromocriptine이 흰쥐 뇌하수체 Prolactin 세포에 미치는 영향에 관한 실험적 연구

Abstract

Hypcrprolactincmia which is produced by pituitary tumor, lesions involving hypothalamo-hypophyscal tract and various drugs has important medical significance because of its close relationship with infertility. Among various drugs which produce hyperprolactincrnia chlorpromazine rapidly elevates blood level of prolactin by blocking the PIF receptor of the pituitary prolactin cells. Bromocriptine is a semisynthetic tripeptide ergot alkaloid and it has high specificity to the prolactin cells. It rapidly lowers blood prolactin level by stimulating the PIF receptor of the prolactin cells at the pituitary level and a few other mechanisms. Furthermore bromocriptine is known to inhibit growth hormone cells directly though still in a controversy. Chlorpromazine and bromocriptine were administered to 120 male rats grouped into 5. Pituitary, adrenals and testes were weighed. Pituitary was prepared for ligh t microscopic, electron microscopic and enzyme cytochemical study. By light microscope, the numbers of the acidophils were counted and sizes were measured for statistical analysis and by electron microscope the ultrastructural changes of the prolactin and growth hormone cells were examined. The results were as follows. 1. By light microscope there was no significant change in numbers of acidophils between each group but there was significant decrease in size of acidophils of combined chlorpromazine and bromocriptine group compared with control and chlorpromazine group. 2. By electron microscope there were severe depletion of prolactin granules and marked development of endoplasmic reticulum and Golgi complex in chlorpromazine group. In combined chlorpromazine and bromocriptine group, there was no significant change of prolactin granules but cellular organelles were markedly decreased in number. With continued bromocriptine administration, cell border and nuclear membrane of the prolactin cell became indistinct and most of the prolactin cells were atrophied with pyknotic nuclei. Growth hormone cells showed no significant changes in each group corn- 316 pared with control group. 3. Enzyme staining of the anterior pituitary showed decreased' activities of NADH and TPPase in chlorpromazine group and combined chlorpromazine and bromocriptine group.