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Development of water-insoluble chitosan patch scaffold to repair traumatic tympanic membrane perforations

Cited 34 time in Web of Science Cited 29 time in Scopus
Authors
Kim, Jang Ho; Bae, Joon-Ho; Lim, Ki Taek; Choi, Seong Jun; Im, Ae Lee; Park, Jung-Sub; Choung, Pill-Hoon; Lee, Eung Tae; Chung, Jong Hoon; Choung, Yun-Hoon
Issue Date
2009-08
Publisher
Wiley-Blackwell
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.90A, No.2, pp.446-455
Keywords
tympanic membraneperforationotitis mediachitosan patch scaffoldhealing
Abstract
Perforated tympanic membranes (TM) and otitis media can be managed with a paper patch or tympanoplasty. However, a paper patch is not biocompatible and tympanoplasty requires complex aseptic surgical procedures. A novel biocompatible patch with a water-insoluble chitosan as the main component was prepared. Optimal mechanical characteristics of a water-insoluble chitosan patch scaffold (CPS) was similar to 40 mu m in thickness, 7 MPa in tensile strength, and 107% in percent elongation, even though the characteristics varied significantly depending on the concentrations of chitosan and glycerol. SEM of the CPSs showed a very smooth surface as compared with that of the paper patches. These CPSs showed no cytotoxicity and had a stimulating effect on the proliferation of TM cells in in vitro study. In in vivo study, 4 (21.19%) and 1.7 (89.5%) TMs out of 19 adult rats with CPSs showed no perforations at 1 and 2 weeks, respectively. However, left control TMs showed healing of 0 (0%) at 1 week and 18 (94.7%) at 2 weeks. TEM findings of regenerated eardrums using CPSs showed thinner, smoother, and more compact tissues than spontaneously healed eardrums. A CPS was more effective than spontaneous healing to repair traumatic TM perforations. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 446-455,2009
ISSN
1549-3296
Language
English
URI
https://hdl.handle.net/10371/80493
DOI
https://doi.org/10.1002/jbm.a.32119
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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