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Development of water-insoluble chitosan patch scaffold to repair traumatic tympanic membrane perforations
Cited 38 time in
Web of Science
Cited 35 time in Scopus
- Authors
- Issue Date
- 2009-08
- Publisher
- Wiley-Blackwell
- Citation
- JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.90A, No.2, pp.446-455
- Keywords
- tympanic membrane ; perforation ; otitis media ; chitosan patch scaffold ; healing
- Abstract
- Perforated tympanic membranes (TM) and otitis media can be managed with a paper patch or tympanoplasty. However, a paper patch is not biocompatible and tympanoplasty requires complex aseptic surgical procedures. A novel biocompatible patch with a water-insoluble chitosan as the main component was prepared. Optimal mechanical characteristics of a water-insoluble chitosan patch scaffold (CPS) was similar to 40 mu m in thickness, 7 MPa in tensile strength, and 107% in percent elongation, even though the characteristics varied significantly depending on the concentrations of chitosan and glycerol. SEM of the CPSs showed a very smooth surface as compared with that of the paper patches. These CPSs showed no cytotoxicity and had a stimulating effect on the proliferation of TM cells in in vitro study. In in vivo study, 4 (21.19%) and 1.7 (89.5%) TMs out of 19 adult rats with CPSs showed no perforations at 1 and 2 weeks, respectively. However, left control TMs showed healing of 0 (0%) at 1 week and 18 (94.7%) at 2 weeks. TEM findings of regenerated eardrums using CPSs showed thinner, smoother, and more compact tissues than spontaneously healed eardrums. A CPS was more effective than spontaneous healing to repair traumatic TM perforations. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 446-455,2009
- ISSN
- 1549-3296
- Language
- English
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