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Extended Exposure to Trichostatin A after Activation Alters the Expression of Genes Important for Early Development in Nuclear Transfer Murine Embryos

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dc.contributor.authorKang, Hoin-
dc.contributor.authorRoh, Sangho-
dc.date.accessioned2013-01-15T00:38:21Z-
dc.date.available2013-01-15T00:38:21Z-
dc.date.issued2011-05-
dc.identifier.citationJOURNAL OF VETERINARY MEDICAL SCIENCE, Vol.73, No.5, pp.623-631ko_KR
dc.identifier.issn0916-7250-
dc.identifier.urihttps://hdl.handle.net/10371/80536-
dc.description.abstractThe low viability of embryos reconstructed by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic modification errors, and reduction of those errors may improve the viability of SCNT embryos. The present study shows the effect of trichostatin A (TSA), a strong inhibitor of histone deacetylase, on the development of murine SCNT embryos. After enucleation and nuclear injection, reconstructed murine oocytes were activated with or without TSA for 6 hr (TSA-6 hr). After activation, TSA treatment was extended to 3 hr (TSA-9 hr), 5 hr (TSA-11 hr) and 18 hr (TSA-24 hr) during culture. As a result, the SCNT embryos in the TSA-11 hr group showed a remarkably higher blastocyst rate (21.1%) when compared with the nontreated embryos (3.4%), while the concentration of TSA did not significantly affect embryonic development. The expressions of histone deacetylase (HDAC1 and HDAC2) and DNA methylation (DNMT3a and DNMT3b) genes decreased in the TSA-11 hr and TSA-24 hr groups, while there was an increase in the expression of histone acetyltransferase (P300 and CBP), pluripotency (OCT4 and NANOG) and embryonic growth/trophectoderm formation (FGF4)-related genes in the same groups. The expression of CDX2, a critical gene for trophectoderm formation was upregulated only in the TSA-24 hr group. Our results show that TSA treatment during the peri- and postactivation period improves the development of reconstructed murine embryos, and this observation may be explained by enhanced epigenetic modification of somatic cells caused by TSA-induced hyperacetylation, demethylation and upregulation of pluripotency and embryonic growth after SCNT.ko_KR
dc.language.isoenko_KR
dc.publisherJAPAN SOC VET SCIko_KR
dc.subjectepigenetic modificationko_KR
dc.subjecttrichostatin Ako_KR
dc.subjectpluripotencyko_KR
dc.subjectsomatic cell nuclear transferko_KR
dc.titleExtended Exposure to Trichostatin A after Activation Alters the Expression of Genes Important for Early Development in Nuclear Transfer Murine Embryosko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor강호인-
dc.contributor.AlternativeAuthor노상호-
dc.identifier.doi10.1292/jvms.10-0492-
dc.citation.journaltitleJOURNAL OF VETERINARY MEDICAL SCIENCE-
dc.description.tc3-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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