S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells
- Kim, Hak-Sung; Kim, Kyoung-Hwa; Kim, Su-Hwan; Kim, Young-Sung; Kim, Tae-Il; Ku, Young; Chung, Chong-Pyoung; Lee, Yong-Moo; Rhyu, In-Chul; Seol, Yang-Jo; Koo, Ki-Tae
- Issue Date
- Journal of Periodontal and Implant Science; Vol.40, No.6, pp.265-270
- Purpose: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells
on allogenic and xenogenic immune cells in vitro.
Methods: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition
of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method.
Results: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic
PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups. Conclusions: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.