S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Experimental salivary pellicles formed on the surface of self-curing resin
- Yoo, J.-H.; Kho, H.-S.; Kim, Y.-K.; Lee, S.-W.; Chung, S.-C.
- Issue Date
- Blackwell Publishing Ltd
- Journal of Oral Rehabilitation 2003 Vol.30, No.3, pp.251-259
- The definitive version is available at www.blackwell-synergy.com
- The aim of this study was to identify the salivary components present in the pellicles formed on self-curing resin and to investigate the qualitative variations in adsorbed salivary pellicle compositions according to different exposure time to saliva. Experimental pellicles were formed by the incubation of polymerized resin particles with fresh human parotid or submandibular-sublingual saliva for either 20 min or 2 h. Pellicles were extracted using formic acid and lyophilized, they were then subjected to sodium dodecyl sulphate–polyacrylamide gel electrophoresis and immunoblotting to identify the adsorbed salivary components. The amino acid proﬁles of the 2 h-pellicles were analysed and compared with those of fresh glandular salivas. There was a difference in the 2 h-pellicle components on the self-curing resin compared with those of other dental materials as well as tooth enamel. The amino acid proﬁles of the 2 h-pellicles were also different from those of fresh glandular salivas. In the case of submandibular-sublingual saliva, the components of the 2 h-pellicle showed a different pattern compared with those of the 20 min-pellicle. However, there was no signiﬁcant difference between the components of the 2 h- and 20 min-pellicles in the case of parotid saliva. A distinct difference was found in the surface binding afﬁnities of immunoglobulin (IgA) from different glandular salivas. The ﬁndings of this study provide information concerning the initial bacterial adhesion on the surfaces of self-curing resin.
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