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Treponema denticola suppresses expression of human beta-defensin-2 in gingival epithelial cells through inhibition of TNF alpha production and TLR2 activation

Cited 25 time in Web of Science Cited 28 time in Scopus
Authors
Shin, Ji Eun; Choi, Youngnim
Issue Date
2010-04
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Citation
MOLECULES AND CELLS; Vol.29, No.4, pp.407-412
Keywords
gingival epitheliumTreponema denticolaTNF alphahuman beta-defensin-2TLR2
Description
The original publication is available at www.springerlink.com
Abstract
We previously reported that Treponema denticola, a periodontal pathogen, suppressed the expression of human beta-defensins (HBDs) and IL-8 in human gingival epithelial cells. To clarify the receptor(s) involved in the suppression of HBD-2, immortalized gingival epithelial (HOK-16B) cells were infected with live or heat-killed T. denticola for 24 h, and the expression of HBD-2 was examined by real-time RT-PCR. Live T. denticola, but not heat-killed bacteria, suppressed the expression of HBD-2 about 40%. Time courses of suppression revealed that T. denticola suppressed HBD-2 expression only at late time points, which was accompanied with the suppression of TNF alpha production. Neutralization of TNF alpha with an antibody abrogated the suppressive effect of T. denticola on HBD-2. Accordingly, heat-killed T. denticola did not suppress TNF alpha production. Knock-down of toll-like receptor (TLR) 2 via RNA interference reversed the suppressive effect of T. denticola on the expression of HBD-3, but not on the production of TNF alpha. Collectively, T. denticola suppresses the expression of HBD-2 in gingival epithelial cells by inhibiting the TLR2 axis and TNF alpha production, which may contribute to the pathogenesis of periodontitis by T. denticola.
ISSN
1016-8478
Language
English
URI
http://hdl.handle.net/10371/80882
DOI
https://doi.org/10.1007/s10059-010-0048-5
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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