Browse

Inhibitory effects of oligonol on phorbol ester-induced tumor promotion and COX-2 expression in mouse skin: NF-κB and C/EBP as potential targets

Cited 30 time in Web of Science Cited 29 time in Scopus
Authors
Kundu, Joydeb Kumar; Hwang, Dal-Mi; Lee, Jung-Chul; Chang, Eun-Jin; Shin, Young Kee; Fujii, Hajime; Sun, Buxiang; Surh, Young-Joon
Issue Date
2009-01
Publisher
ELSEVIER IRELAND LTD
Citation
Cancer Letters, Vol.273 No.1, pp.86-97
Keywords
OligonolChemopreventionMouse skinCyclooxygenase-2MAPKNF-kappa BC/EBP
Abstract
Plant polyphenols possess anti-oxidant and anti-inflammatory activities and are hence potential candidates for preventing cancer. The present study was aimed at evaluating the anti-inflammatory and anti-tumor promoting activity of oligonol, a formulation of catechin-type oligomers, in mouse skin stimulated with a proto-type tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Pretreatment of mouse skin with oligonol significantly inhibited TPA-induced expression of cyclooxygenase-2 (COX-2). Oligonol diminished nuclear translocation and DNA binding of nuclear factor-kappaB (NF-kappa B) via blockade of phosphorylation and subsequent degradation of I kappa B alpha in TPA-treated mouse skin. Moreover, oligonol suppressed TPA-induced DNA binding of CCAAT/enhancer-binding protein (C/EBP) in mouse skin. Oligonol pretreatment also attenuated the phosphorylation and/or catalytic activities of extracellular signal-regulated protein kinase-1/2 (ERK1/2) and p38 mitogen-activated protein (MAP) kinase. Moreover, p38 MAP kinase inhibitor SB203580, but not the MEK inhibitor U0126, negated TPA-induced DNA binding of C/EBP. In addition, oligonol reduced the incidence and the multiplicity of papillomas and squamous cell carcinomas in 7,12-dimethylbenz[a]anthracene (DMBA)-initiated and TPA-promoted mouse skin, and prolonged the survival of tumor-bearing mice. Pretreatment with oligonol diminished the levels of proliferating cell nuclear antigen and expression of COX-2 in papillomas and carcinomas, respectively, as compared to DMBA plus TPA treatment alone. Taken together, the above findings suggest that oligonol inhibits TPA-induced COX-2 expression by blocking the activation of NF-kappa B and C/EBP via modulation of MAP kinases and suppresses chemically induced mouse skin tumorigenesis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
ISSN
0304-3835
Language
English
URI
http://hdl.handle.net/10371/80940
DOI
https://doi.org/10.1016/j.canlet.2008.07.039
Files in This Item:
There are no files associated with this item.
Appears in Collections:
Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse