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Identification of formaldehyde-responsive genes by suppression subtractive hybridization

Cited 7 time in Web of Science Cited 10 time in Scopus
Authors
Lee, Min-Ho; Kim, Young-Ae; Na, Tae-Young; Lee, Byung-Hoon; Shin, Young Kee; Kim, Sung-Hye; Shin, Ho-Sang; Lee, Mi-Ock
Issue Date
2008-01-14
Publisher
ELSEVIER IRELAND LTD
Citation
TOXICOLOGY; Vol.243, pp.224-235
Keywords
formaldehydesubtractive hybridizationMDM2calcyclin
Abstract
Formaldehyde is frequently used in indoor household and occupational environments. Inhalation of formaldehyde invokes an inflammatory response, including a variety of allergic signs and symptoms. Therefore, formaldehyde has been considered as the most prevalent cause of sick building syndrome, which has become a major social problem, especially in developing urban areas. Further formaldehyde is classified as a genotoxicant in the respiratory tract of rats and humans. To better understand the molecular mechanisms involved in formaldehyde intoxication, we sought differentially regulated genes by formaldehyde exposure to Hs 680.Tr human trachea cells, using polymerase chain reaction (PCR)-based suppression subtractive hybridization. We identified 27 different formaldehyde-inducible genes, including those coding for the major histocompatibility complex, class IA, calcyclin, glutathione S-transferase pi, mouse double minute 2 (MDM2), platelet-derived growth factor receptor alpha, and which are known to be associated with cell proliferation and differentiation, immunity and inflammation, and detoxification. Induction of these genes by formaldehyde treatment was confirmed by reverse transcription PCR and western blot analysis. Further, the expression of calcyclin, glutathione S-transferase pi, PDGFRA and MDM2 were significantly induced in the trachea] epithelium of Sprague Dawley rats after formaldehyde inhalation. Our results suggest that the elevated levels of these genes may be associated with the formaldehyde-induced toxicity, and that they deserve evaluation as potential biomarkers for formaldehyde intoxication. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
ISSN
0300-483X
Language
English
URI
https://hdl.handle.net/10371/80941
DOI
https://doi.org/10.1016/j.tox.2007.10.007
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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