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Expression profile of the stem cell markers in human hertwig`s epithelial root sheath/Epithelial rests of Malassez cells

Cited 33 time in Web of Science Cited 36 time in Scopus
Authors
Nam H.; Kim J.; Park J.; Park J.-C.; Seo B.-M.; Lee G.; Lee J.C.; Kim J.-W.
Issue Date
2011
Publisher
Korean Society for Molecular and Cellular Biology
Citation
Molecules and Cells, Vol.31, No.4, pp.355-360
Keywords
Embryonic stem cellsEpithelial stem cellsHertwig`s epithelial root sheath/Epithelial rests of Malassez cellsStem cell markers
Abstract
Hertwig`s epithelial root sheath/Epithelial rests of Malassez (HERS/ERM) cells are unique epithelial cells in the periodontal ligament. They remain in periodontal tissues throughout the adult life, and it is expected that their functional role is to maintain the homeostasis of the periodontium through reciprocal interactions with other periodontal cells. In this study, we investigated whether HERS/ERM cells have primitive stem cell characteristics: those of embryonic stem cells as well as of epithelial stem cells. Primary HERS/ERM cells had typical epithelial cell morphology and characteristics and they maintained for more than five passages. They expressed epithelial stem cell-related genes: ABCG2, ?Np63, p75, EpCAM, and Bmi-1. Moreover, the expression of embryonic stem cell markers such as Oct-4, Nanog, and SSEA-4 were detected. Next, we investigated whether the expression of these stem cell markers was maintained during the sub-culture process. HERS/ERM cells showed different expression levels of these stemness genes at each passage, but their expression was maintained throughout the passages. Taken together, our data suggest that a primary culture of HERS/ERM cells contains a population of primitive stem cells that express epithelial stem cell markers and embryonic stem cell markers. Furthermore, these cell populations were maintained during the sub-culturing process in our culture conditions. Therefore, our findings suggest that there is a strong possibility of accomplishing cementum tissue engineering with HERS/ERM cells.
ISSN
1016-8478
Language
English
URI
http://hdl.handle.net/10371/80942
DOI
https://doi.org/10.1007/s10059-011-0045-3
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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