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Initial adhesion of bone marrow stromal cells to various bone graft substitutes

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Jo, Young-Jae; Kim, Kyoung-Hwa; Koo,Ki-Tae; Kim, Tae-Il; Lee, Yong-Moo; Chung, Chong-Pyoung; Rhyu, In-Chul; Ku, Young; Seol, Yang-Jo
Issue Date
2011
Publisher
Korean Academy of Periodontology
Citation
Journal of Periodontal and Implant Science, Vol.41, No.2, pp.67-72
Keywords
Bone substitutesCell adhesionStem cellsFibronectins
Abstract
Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with beta-tricalcium phosphate (TCP), and pure beta-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7. Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface. Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment. (C) 2011 Korean Academy of Periodontology.
ISSN
2093-2278
Language
English
URI
https://hdl.handle.net/10371/80944
DOI
https://doi.org/10.5051/jpis.2011.41.2.67
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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