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The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects
DC Field | Value | Language |
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dc.contributor.author | Park, Shin-Young | - |
dc.contributor.author | Kim, Kyoung-Hwa | - |
dc.contributor.author | Koo, Ki-Tae | - |
dc.contributor.author | Lee, Kang-Woon | - |
dc.contributor.author | Chung, Chong-Pyoung | - |
dc.contributor.author | Seol, Yang-Jo | - |
dc.contributor.author | Lee, Yong-Moo | - |
dc.date.accessioned | 2013-01-23T07:22:31Z | - |
dc.date.available | 2013-01-23T07:22:31Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Journal of Periodontal and Implant Science, Vol.41, No.5, pp.218-226 | ko_KR |
dc.identifier.issn | 2093-2278 | - |
dc.identifier.uri | https://hdl.handle.net/10371/81050 | - |
dc.description.abstract | Purpose: Micro-computed tomography (micro-CT) has been widely used in the evaluation of regenerated bone tissue but the reliability of micro-CT has not yet been established. This study evaluated the correlation between histomorphometric analysis and micro-CT analysis in performing new bone formation measurement. Methods: Critical-size calvarial defects were created using a 8 mm trephine bur in a total of 24 Sprague-Dawley rats, and collagen gel mixed with autogenous rat bone marrow stromal cells (BMSCs) or autogenous rat BMSCs transduced by adenovirus containing bone morphogenic protein-2 (BMP-2) genes was loaded into the defect site. In the control group, collagen gel alone was loaded into the defect. After 2 and 4 weeks, the animals were euthanized and calvaria containing defects were harvested. Micro-CT analysis and histomorphometric analysis of each sample were accomplished and the statistical evaluation about the correlation between both analyses was performed. Results: New bone formation of the BMP-2 group was greater than that of the other groups at 2 and 4 weeks in both histomorphometric analysis and micro-CT analysis (P=0.026, P=0.034). Histomorphometric analysis of representative sections showed similar results to histomorphometric analysis with a mean value of 3 sections. Measurement of new bone formation was highly correlated between histomorphometric analysis and micro-CT analysis, especially at the low lower threshold level at 2 weeks (adjusted r 2=0.907, P<0.001). New bone formation of the BMP-2 group analyzed by micro-CT tended to decline sharply with an increasing lower threshold level, and it was statistically significant (P<0.001). Conclusions: Both histomorphometric analysis and micro-CT analysis were valid methods for measurement of the new bone in rat calvarial defects and the ability to detect the new bone in micro-CT analysis was highly influenced by the threshold level in the BMP-2 group at early stage. (C) 2011 Korean Academy of Periodontology. | ko_KR |
dc.description.sponsorship | This study was supported by a grant of the Korean Health Technology R&D Projects, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A085056). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Korean Academy of Periodontology | ko_KR |
dc.subject | Bone morphogenic protein-2 | ko_KR |
dc.subject | Gene therapy | ko_KR |
dc.subject | Histology | ko_KR |
dc.subject | X-ray microtomography | ko_KR |
dc.title | The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박신영 | - |
dc.contributor.AlternativeAuthor | 김경화 | - |
dc.contributor.AlternativeAuthor | 구기태 | - |
dc.contributor.AlternativeAuthor | 이강원 | - |
dc.contributor.AlternativeAuthor | 정종평 | - |
dc.contributor.AlternativeAuthor | 설양조 | - |
dc.contributor.AlternativeAuthor | 이용무 | - |
dc.identifier.doi | 10.5051/jpis.2011.41.5.218 | - |
dc.citation.journaltitle | Journal of Periodontal and Implant Science | - |
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