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Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow

Cited 25 time in Web of Science Cited 27 time in Scopus
Authors
Kim, Su-Hwan; Kim, Young-Sung; Lee, Su-Yeon; Kim, Kyoung-Hwa; Kim, Won-Kyung; Lee, Young-Kyoo; Lee, Yong-Moo
Issue Date
2011
Publisher
Korean Academy of Periodontology
Citation
Journal of Periodontal and Implant Science; Vol.41, No.4, pp.192-200
Keywords
Adult stem cellsmRNAGene expression profilingMesenchymal stem cellsMicroarray analysis
Abstract
Purpose: The aim of this study is to compare the gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow for characterization of dental stem cells. Methods: We employed GeneChip analysis to the expression levels of approximately 32,321 kinds of transcripts in 5 samples of bone-marrow-derived mesenchymal stem cells (BMSCs) (n=1), periodontal ligament stem cells (PDLSCs) (n=2), and dental pulp stem cells (DPSCs) (n=2). Each cell was sorted by a FACS Vantage Sorter using immunocytochemical staining of the early mesenchymal stem cell surface marker STRO-1 before the microarray analysis. Results: We identified 379 up-regulated and 133 down-regulated transcripts in BMSCs, 68 up-regulated and 64 down-regulated transcripts in PDLSCs, and 218 up-regulated and 231 down-regulated transcripts in DPSCs. In addition, anatomical structure development and anatomical structure morphogenesis gene ontology (GO) terms were over-represented in all three different mesenchymal stem cells and GO terms related to blood vessels, and neurons were over-represented only in DPSCs. Conclusions: This study demonstrated the genome-wide gene expression patterns of STRO-1 + mesenchymal stem cells derived from dental tissues and bone marrow. The differences among the expression profiles of BMSCs, PDLSCs, and DPSCs were shown, and 999 candidate genes were found to be definitely up- or down-regulated. In addition, GOstat analyses of regulated gene products provided over-represented GO classes. These data provide a first step for discovering molecules key to the characteristics of dental stem cells. ⓒ 2011 Korean Academy of Periodontology.
ISSN
2093-2278
Language
English
URI
https://hdl.handle.net/10371/81061
DOI
https://doi.org/10.5051/jpis.2011.41.4.192
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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