Effects of epidural TNF-alpha inhibitor injection: analysis of the pathological changes in a rat model of chronic compression of the dorsal root ganglion

Abstract

To evaluate the efficacy of a single direct epidural injection of tumor necrosis factor (TNF)-alpha inhibitor to reduce the pathological changes in nerve fiber injuries in a rat model of chronic compression of the dorsal root ganglion (CCD).A surgical procedure for CCD was performed in 17 adult male F-344 rats. The effects of the epidural TNF-alpha inhibitors on CCD-induced pathological changes were investigated. Three groups of rats (n = 17) were used: (1) CCD + saline (n = 4), (2) CCD + triamcinolone (n = 5), and (3) CCD + TNF-alpha inhibitors (n = 8). Their dorsal root ganglia and nerve roots were removed on postoperative day 14. The intraneural edema, demyelination, and Wallerian degeneration of all 17 rats were scored pathologically.The pathology scores of the rats in the TNF-alpha inhibitor treatment group (1.38 +/- 0.74) indicated a mild degree of intraneural edema compared to the saline treatment group (2.25 +/- 0.50, p = 0.041). In addition, rats in the TNF-alpha inhibitor treatment group (2.13 +/- 0.35) had a mild degree of demyelination compared to the saline treatment group (2.75 +/- 0.50, p = 0.038) and the triamcinolone treatment group (2.80 +/- 0.45, p = 0.019). The differences in the pathology scores for Wallerian degeneration were not statistically significant in all three study groups (p = 0.658).The epidural injection of a TNF-alpha inhibitor was more effective than a placebo and comparable to triamcinolone in reducing pathological nerve injury progression.

Objectives To evaluate the efficacy of a single direct epidural injection of tumor necrosis factor (TNF)-α inhibitor to reduce the pathological changes in nerve fiber injuries in a rat model of chronic compression of the dorsal root ganglion (CCD). Materials and methods A surgical procedure for CCD was performed in 17 adult male F-344 rats. The effects of the epidural TNF-α inhibitors on CCD-induced pathological changes were investigated. Three groups of rats (n=17) were used: (1) CCD+saline (n=4), (2) CCD+triamcinolone (n=5), and (3) CCD+TNF-α inhibitors (n=8). Their dorsal root ganglia and nerve roots were removed on postoperative day 14. The intraneural edema, demyelination, andWallerian degeneration of all 17 rats were scored pathologically. Results The pathology scores of the rats in the TNF-α inhibitor treatment group (1.38±0.74) indicated a mild degree of intraneural edema compared to the saline treatment group (2.25±0.50, p=0.041). In addition, rats in the TNF-α inhibitor treatment group (2.13±0.35) had a mild degree of demyelination compared to the saline treatment group (2.75±0.50, p=0.038) and the triamcinolone treatment group (2.80±0.45, p=0.019). The differences in the pathology scores for Wallerian degeneration were not statistically significant in all three study groups (p=0.658). Conclusion The epidural injection of a TNF-α inhibitor was more effective than a placebo and comparable to triamcinolone in reducing pathological nerve injury progression.