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The Changes in MGMT Promoter Methylation Status in Initial and Recurrent Glioblastomas

Cited 34 time in Web of Science Cited 36 time in Scopus
Issue Date
2012-10
Publisher
SPRINGER
Citation
CLINICAL & TRANSLATIONAL ONCOLOGY Vol.5 No.5, pp. 393-397
Keywords
복합학
Abstract
To evaluate the mechanism of the development of therapeutic resistance after temozolomide treatment, we focused on changes in O-6-methylguanine DNA methyltransferase (MGMT) and mismatch repair (MMR) between initial and recurrent glioblastomas. Tissue samples obtained from 24 paired histologically confirmed initial and recurrent adult glioblastoma patients who were initially treated with temozolomide were used for MGMT and MMR gene promoter methylation status and protein expression analysis using methylation-specific multiplex ligation probe amplification (MS-MLPA), methylation-specific polymerase chain reaction (MSP), and immunohistochemical staining. There was a significant decrease in the methylation ratio of the MGMT promoter determined by MS-MLPA, which was not detectable with MSP, and MGMT protein expression changes were not remarkable. However, there was no epigenetic variability in MMR genes, and a relatively homogeneous expression of MMR proteins was observed in initial and recurrent tumors. We conclude that the development of reduced methylation in the MGMT promoter is one of the mechanisms for acquiring therapeutic resistance after temozolomide treatment in glioblastomas.
ISSN
1699-048X
Language
English
URI
https://hdl.handle.net/10371/81385
DOI
https://doi.org/10.1593/tlo.12253
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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