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Odontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factors

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dc.contributor.authorLee, Ji-Hyun-
dc.contributor.authorLee, Dong-Seol-
dc.contributor.authorChoung, Han-Wool-
dc.contributor.authorShon, Won-Jun-
dc.contributor.authorSeo, Byoung-Moo-
dc.contributor.authorLee, Eun-Hyang-
dc.contributor.authorCho, Je-Yoel-
dc.contributor.authorPark, Joo-Cheol-
dc.creator박주철-
dc.date.accessioned2013-03-22T01:17:55Z-
dc.date.available2013-03-22T01:17:55Z-
dc.date.issued2011-12-
dc.identifier.citationBIOMATERIALS Vol.32 No.36, pp. 9696-9706-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/10371/81391-
dc.description.abstractThe differentiation of odontoblasts is initiated by the organization of differentiating ameloblasts during tooth formation. However, the exact roles of ameloblast-derived factors in odontoblast differentiation have not yet been characterized. We investigated the effects of preameloblast-conditioned medium (PACM) on the odontogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro and in vivo. Furthermore, we analyzed the PA-CM by liquid chromatography-mass spectrometry to identify novel factors that facilitate odontoblast differentiation. In the co-culture of MDPC-23 cells or hDPSCs with mouse apical bud cells (ABCs), ABCs promoted differentiation of odontoblastic MDPC-23 cells and facilitated odontoblast differentiation of hDPSCs. PA-CM, CM from ABCs after 3 days culture, was most effective in increasing the dentin sialophosphoprotein promoter activity of odontoblastic MDPC-23 cells. When PA-CM-treated hDPSCs were transplanted into immunocompromised mice, they generated pulp-like structures lined with human odontoblast-like cells showing typical odontoblast processes. However, during recombinant human bone morphogenenetic protein 2-treated hDPSCs transplantation, some of the cells were entrapped in mineralized matrix possessing osteocyte characteristics. After proteomic analyses, we identified 113 types of proteins in PA-CM, of which we characterized 23. The results show that preameloblast-derived factors induce the odontogenic differentiation of hDPSCs and promote dentin formation. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.language.isoen-
dc.publisherELSEVIER SCI LTDen
dc.subject복합학en
dc.subjectEpithelial-mesenchymal interaction-
dc.subjectPreameloblast-CM-
dc.subjectHuman dental pulp stem cell-
dc.subjectOdontoblast differentiation-
dc.subjectDentin regeneration-
dc.titleOdontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factorsen
dc.typeArticle-
dc.contributor.AlternativeAuthor이지현-
dc.contributor.AlternativeAuthor이동설-
dc.contributor.AlternativeAuthor정한울-
dc.contributor.AlternativeAuthor손원준-
dc.contributor.AlternativeAuthor서병무-
dc.contributor.AlternativeAuthor이은향-
dc.contributor.AlternativeAuthor조제열-
dc.contributor.AlternativeAuthor박주철-
dc.identifier.doi10.1016/j.biomaterials.2011.09.007-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2011-01/102/0000042428/5-
dc.description.srndSEQ:5-
dc.description.srndPERF_CD:SNU2011-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000042428-
dc.description.srndADJUST_YN:Y-
dc.description.srndEMP_ID:A077448-
dc.description.srndDEPT_CD:861-
dc.description.srndCITE_RATE:7.404-
dc.description.srndFILENAME:Biomaterials-online version-CR.pdf-
dc.description.srndDEPT_NM:치의학과-
dc.description.srndEMAIL:jcapark@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2011-01/102/0000042428/5-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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