S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Journal Papers (저널논문_의학과)
Association of Polymorphism in Pri-microRNAs-371-372-373 with the Occurrence of Hepatocellular Carcinoma in Hepatitis B Virus Infected Patients
- Kwak, Min-Sun; Lee, Dong Hyeon; Cho, Yuri; Cho, Eun-Ju; Lee, Jeong-Hoon; Yu, Su Jong; Yoon, Jung-Hwan; Lee, Hyo-Suk; Kim, Chung Yong; Cheong, Jae Youn; Cho, Sung Won; Shin, Hyoung Doo; Kim, Yoon Jun
- Issue Date
- PUBLIC LIBRARY OF SCIENCE
- PLOS ONE Vol.7 No.7, pp. e41983
- Background: Micro RNAs-371-372-373 (miRNAs-371-373), originating from the same pri-miRNA transcript, are reported to
be upregulated in hepatocellular carcinoma (HCC) and to be related to the regulation of hepatitis B virus (HBV) infection.
Our study investigated whether pri-miRNAs-371-373 polymorphisms are associated with the risk of HCC occurrence and
Methods: Genetic variations were identified through direct DNA sequencing using TaqMan assay. Three sequence variants
of pri-miRNAs-371-373 were identified. Genetic associations of those with HCC occurrence and HBV clearance among
patients with HBV infection were analyzed using logistic regression analyses with adjustment for age and gender (n = 1439).
Results: For the occurrence of HCC, polymorphism rs3859501C.A acted as a protective factor both in chronic carriers
(OR = 0.75, P = 0.005 in a codominant model; OR = 0.71, P = 0.02 in a dominant model; OR = 0.66, P = 0.03 in recessive model)
and liver cirrhosis patients (OR = 0.69, P = 0.001 in a codominant model; OR = 0.60, P = 0.003 in a dominant model; OR = 0.63,
P = 0.03 in a recessive model). The pri-miRNAs-371-373_ht2 [C-A-C] also showed a protective effect on HCC occurrence both
in the chronic carrier and liver cirrhosis groups (P,0.05 in both). However, there was no significant association between primiRNAs-
371-373 polymorphisms and HBV clearance.
Conclusions: In conclusion, among chronic carriers and liver cirrhosis patients, the A allele of rs3859501 and the haplotype
pri-miRNAs-371-373_ht2 were more protective to HCC than other genotypes and haplotypes. Further studies into the roles
of rs3859501 and pri-miRNAs-371-373_ht2 haplotype in hepatocarcinogenesis are needed.