납 이온이 토끼 적혈구의 포타슘 투과에 미치는 영향

Abstract

The effects of lead on the permeability of rabbit red cells to potassium were studied by analyzing doseresponse, time·responese curves and osmotic fragility changes at varying times. Other metal cations. such as mercury, copper and zine were also tested. The following results were obtained. 1) Lead chloride caused loss of K+ from rabbit red cells at concentrations ranging between O.5X10-3 and 20X 10-3 mM. The Krloss progressed rapidly for about 60 minutes. but thereafter slowed down. lead chloride induced a prehemolytic loss of K" from the red cells over a range from 0.5 X 10-3mM to 20X 10-3 mM/L PbCl z• and at concentration above 20X lO<J mlvl, hemolysis began to appear. 2) The lead induced a loss of K+from rabbit red cells as an ali-or-none response of individual cells within the population. 3) As the time elapsed after lead poisoning on red cells. a recovery of cell membrane was observed. It took five hours for the cell membrane to recover. 4) Factors which influence the loss of K+ from the Pb poisoned rabbit red cells were pH and temperature. The maximum permeability to K+ occured between 20' and 37°C and there was an optimum pH for penetration of K+ in the region of 7. O. 5) The formation of intracellular inorganic phosphate in t he lead poisoned rabbit red cell was augmented greatly in the rapid K+ loss phase. and thereafter slowed down sligtly, 6) Mercury and lead induced K+ leakage in rabbit red cells, but zinc didn't, while copper had a much smaller eft ect. 7) The relation between sulf hydryl group and Pb binding on red cell membrane was discussed.