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Cell Adhesion-Dependent Serine 85 Phosphorylation of Paxillin Modulates Focal Adhesion Formation and Haptotactic Migration via an Association with the C-Terminal Tail Domain of Talin.

Cited 12 time in Web of Science Cited 12 time in Scopus
Issue Date
2012-08
Publisher
American Society for Biochemistry and Molecular Biology
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY Vol.287 No.33, pp. 27499-27509
Keywords
복합학cell adhesionpaxillintalinphosphorlation
Abstract
Integrin-mediated adhesion to extracellular matrix proteins
is dynamically regulated during morphological changes and cell
migration. Upon cell adhesion, protein-protein interactions
among molecules at focal adhesions (FAs) play major roles in the
regulation of cell morphogenesis and migration. Although tyrosine
phosphorylation of paxillin is critically involved in adhesion-
mediated signaling, the significance of paxillin phosphorylation
at Ser-85 and the mechanism by which it regulates cell
migration remain unclear. In this study, we examined how
Ser-85 phosphorylation of paxillin affects FA formation and cell
migration. We found that paxillin phosphorylation at Ser-85
occurred during HeLa cell adhesion to collagen I and was concomitant
with tyrosine phosphorylation of both focal adhesion
kinase and talin. However, the non-phosphorylatable S85A
mutant of paxillin impaired cell spreading, FA turnover, and
migration toward collagen I but not toward serum. Furthermore,
whereas the (presumably indirect) interaction between
paxillin and the C-terminal tail of talin led to dynamic FAs at the
cell boundary, S85A paxillin did not bind talin and caused stabilized
FAs in the central region of cells. Together, these observations
suggest that cell adhesion-dependent Ser-85 phosphorylation
of paxillin is important for its interaction with talin and
regulation of dynamic FAs and cell migration.
ISSN
0021-9258
Language
English
URI
https://hdl.handle.net/10371/82067
DOI
https://doi.org/10.1074/jbc.M111.323360
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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