Initial Osteoblast-like Cell Response to Titanium Coated with Recombinant Human Bone Morphogenic Protein-2 (rhBMP-2)

Abstract

The aim of this study was to investigate the initial bone cell response to titanium discs coated with a biodegradable polymer, poly(D,L-lactide-co-glycolide) (PLGA) incorporated recombinant human Bone Morphogenic Protein-2(rhBMP-2). Titanium discs were fabricated and divided into three groups. Group I was anodized under 300 V and not coated. Group 2 was machined and coated with PLGA/rhBMP-2 (500 ng/ml) solutions. Group 3 was anodized then coated with the same solution. Surface topography was examined by scanning electron microscopy (SEM). The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured. MTS based cell proliferation assay for 1, 3 days and alkaline phosphatase activity test were carried out for 3 and 7 days. It was observed that PLGA/rhBMP particles were observed as smooth and round onto the coated titanium surfaces. Cell proliferation and ALP activity were the highest on anodized and coated Ti surfaces (group 3). In this study, biodegradable PLGA polymers incorporated with rhBMP could enhance proliferation and differentiation of cells on the titanium surface.