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α-Tocopheryl Succinate Inhibits Osteoclast Formation by Suppressing Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL) Expression and Bone Resorption
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ha-Neui | - |
dc.contributor.author | Lee, Jong-Ho | - |
dc.contributor.author | Jin, Won Jong | - |
dc.contributor.author | Lee, Zang Hee | - |
dc.creator | 이장희 | - |
dc.date.accessioned | 2013-05-01T06:31:34Z | - |
dc.date.available | 2013-05-01T06:31:34Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | JOURNAL OF BONE METABOLISM Vol.19 No.2, pp. 111-120 | - |
dc.identifier.issn | 1598-107X | - |
dc.identifier.uri | https://hdl.handle.net/10371/82377 | - |
dc.description.abstract | Objective: Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte/macrophage lineage during
normal and pathological bone turnover. Recently, several studies revealed that alpha-tocopheryl succinate (αTP-suc) have demonstrated potent anti-cancer activities in vitro and in vivo. However, the effects of αTP-suc on osteoclast formation and bone resorption remain unknown. Thus, in this study, we examined the effects of αTP-suc on osteoclast differentiation and bone resorbing activity in inflammatory bone loss model. Methods: Osteoclast differentiation assay was performed by cocultures of mouse bone marrow cells and calvarial osteoblasts in culture media including interleukin-1 (IL-1). Osteoclasts were stained for tartrate-resistant acid phosphatase (TRAP). The level of receptor activator of nuclear factor-kappaB ligand (RANKL) mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). ICR mice were administered an intraperitoneal injections of αTP-suc or dimethyl sulfoxide (DMSO) 1 day before the implantation of a freeze-dried collagen sponge loaded with phosphate-buffered saline (PBS) or IL-1 over the calvariae and every other day for 7 days. The whole calvariae were obtained and analyzed by micro-computed tomography (CT) scanning, and stained for TRAP. Results: αTP-suc inhibits osteoclast formation in cocultures stimulated by IL-1 and decreased the level of expression of RANKL mRNA in osteoblasts. In addition, administered intraperitoneal injections of αTP-suc prevented IL-1-mediated osteoclast formation and bone loss in vivo. Conclusion: Our findings suggest that αTP-suc may have therapeutic value for treating and preventing bone-resorptive diseases, such as osteoporosis. | - |
dc.language.iso | en | en |
dc.publisher | Korean Society for Bone and Mineral Research | en |
dc.subject | 의약학 | en |
dc.subject | Alpha-tocopheryl succinate | - |
dc.subject | Interleukin-1 | - |
dc.subject | Osteoclast | - |
dc.subject | Osteoporosis | - |
dc.subject | RANKL | - |
dc.title | α-Tocopheryl Succinate Inhibits Osteoclast Formation by Suppressing Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL) Expression and Bone Resorption | en |
dc.type | Article | - |
dc.author.alternative | 김하늬 | - |
dc.author.alternative | 이종호 | - |
dc.author.alternative | 진원종 | - |
dc.author.alternative | 이장희 | - |
dc.identifier.doi | 10.11005/jbm.2012.19.2.111 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000026258/9 | - |
dc.description.srnd | SEQ:9 | - |
dc.description.srnd | PERF_CD:SNU2012-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000026258 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A076310 | - |
dc.description.srnd | DEPT_CD:861 | - |
dc.description.srnd | CITE_RATE:0 | - |
dc.description.srnd | FILENAME:골대사학회지-TP-succinate.pdf | - |
dc.description.srnd | DEPT_NM:치의학과 | - |
dc.description.srnd | EMAIL:zang1959@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:N | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2012-01/102/0000026258/9 | - |
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