간흡충에 대한 조직화학적 연구

Abstract

Histochemical studies on the liver fluke, Clonorchis sinensis(Cobbold, 1875), were carried out as - one of the histophysiological studies of the liver flukes. Surveys were made on the distribution of nucleic acid. phosphatase, glycogen, fatty substances, and incrganic subances. 1. Inorganic substances Inorganic iron is found abundantly in the intestine. and it is shown weakly in the cuticula and reticular tissue. Magnesium is not found. Potassium is shown strongly in the female reproductive organ. (uterus, vitelline glands, and eggs) Calcium is shown prominently in the reticular tissue, cuticula, epithelium of the intestine, testes and overy, 2. Glycogen 1) Glycogen is richly found in the reticular tissue, vitelline glands and eggs in the uterus. 2) Polysaccharides aside from glycogen are observed in the subcuticular muscle cells, overy, testes and eggs in the uterus. 3. Nucleic acids 1) Ribonucleic acid is found abundantly in the vitelline glands, ovary, testes and eggs and it is shown weakly in subcuticular muscle cells, epithelium of the intestine and uterus. 2) Desoxyribonucle ic acid is richly found in the testes and ovary. 4. Phosphatase 1) Alkaline phosphatese is detected most prominently in the epithelium of the intestine. epithelium of the uterus, excretory sac, testes and vitelline glands. 2) Acid phosphatase is shown most strongly in the oral sucker, pharynx, esophagus, subcuticular muscle cells, vitelline glands and epitheliun of the intestine. 5. Fatty substances 1) Fatty substances are prominently found in the reticular tissue, subcuticular muscle cells. 2) Fatty substances also are found abundantly in the oral sucker and intestine. 3) It is shown weakly in the reproductive organs. The author discussed about the relations among carbohydrates, nucleic acids, phosphatase and inorganic substances in the metabolic process of the worm.