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Detection by In-situ Hybridization of Pasteurella multocida Toxin (toxA) Gene in the Lungs of Naturally Infected Pigs

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dc.contributor.authorAhn, K.K.-
dc.contributor.authorLee, Y.H.-
dc.contributor.authorHa, Y.-
dc.contributor.authorKim, D.-
dc.contributor.authorChae, S.-
dc.contributor.authorKim, C.H.-
dc.contributor.authorLee, J.-H.-
dc.contributor.authorKim, S.-H.-
dc.contributor.authorChae, Chan Hee-
dc.date.accessioned2009-08-31T23:24:35Z-
dc.date.available2009-08-31T23:24:35Z-
dc.date.issued2008-05-20-
dc.identifier.citationJournal of Comparative Pathology, 139, 51-53en
dc.identifier.issn0021-9975-
dc.identifier.urihttps://hdl.handle.net/10371/8276-
dc.description.abstractIn-situ hybridization with a non-radioactive digoxigenin-labelled probe was used to detect the Pasteurella multocida toxin (PMT) gene in tissue sections of pneumonic lung from pigs naturally infected with toxigenic P. multocida. The morphology of host cells was preserved despite the relatively high temperature used in the incubation procedure. Pulmonary abscessation was observed in 13 pigs naturally infected with toxigenic P. multocida type A (three pigs) or D (10 pigs). In these 13 pigs a strong hybridization signal for PMT DNA was detected, mainly in degenerate leucocytes in abscesses. Occasionally, PMT DNA was detected in degenerate neutrophils and macrophages in alveolar spaces. Detection of hybridization signals for PMT DNA would seem to be a potential indicator of the production of PMT. The study suggested that PMT plays an important role in pulmonary abscessation caused by P. multocida.en
dc.description.sponsorshipThis research was supported by the contract research funds of the Research Institute for Veterinary Science (RIVS) from the College of Veterinary Medicine, Seoul National University, and the Brain Korea 21 Program for Veterinary Science, Republic of Korea.en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectbacterial infectionen
dc.subjectPasteurella multocidaen
dc.subjectpigen
dc.subjectpulmonary abscessen
dc.subjecttoxA geneen
dc.titleDetection by In-situ Hybridization of Pasteurella multocida Toxin (toxA) Gene in the Lungs of Naturally Infected Pigsen
dc.typeArticleen
dc.contributor.AlternativeAuthor채찬희-
dc.identifier.doi10.1016/j.jcpa.2008.03.002-
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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