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One-step multiplex RT-PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual priming oligonucleotide (DPO) system

Cited 30 time in Web of Science Cited 31 time in Scopus
Authors
Lee, C.S.; Kang, B.K.; Lee, D.H.; Lyou, S.H.; Park, BongKyun; Ann, S.K.; Jung, K.
Issue Date
2008-05-19
Publisher
Elsevier
Citation
J. Virol. Methods 151, 30-34
Keywords
Swine influenzavirusMultiplex PCRDPO
Abstract
The swine influenza virus (SIV) H1N1, H1N2, and H3N2 subtypes circulate in Korean farm. A novel multiplex
RT-PCR (m-RT-PCR) was developed to detect and subtype swine influenza viruses. This m-RT-PCR
assay could identify H1, H3, N1 and N2 from clinical samples in single tube reaction using DPO system.
Korean SIVs are closely related to the United States influenza viruses, and primers were developed
for SIV from North American viruses and recently Korean isolates. The sensitivity of the m-RT-PCR was
10TCID50/ml for H1N1, H1N2 or H3N2. The lowest viral concentrations detected by single PCR were
1TCID50/ml for each subtype. Non-specific reactions were not observed when other viruses and bacteria
were used to assess the m-RT-PCR. The results of m-RT-PCR were more effective than virus isolation or
hemagglutination (HA) test. This assay using a DPO system provides a rapid, sensitive, and cost-effective
laboratory diagnosis for detecting and subtyping of SIV in pigs.
ISSN
0166-0934
Language
English
URI
https://hdl.handle.net/10371/8282
DOI
https://doi.org/10.1016/j.jviromet.2008.04.001
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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