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Evaluation of Potential Biomarkers for Thioacetamide-induced Hepatotoxicity using siRNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Jin Seok | - |
dc.contributor.author | Yum, Young Na | - |
dc.contributor.author | Han, Eul Sik | - |
dc.contributor.author | Kim, Joo Hwan | - |
dc.contributor.author | Lee, Eun Mi | - |
dc.contributor.author | Ryu, Doug Young | - |
dc.contributor.author | Kim, Young Hee | - |
dc.contributor.author | Yang, Sung Hee | - |
dc.contributor.author | Kim, Seung Hee | - |
dc.contributor.author | Park, Sue Nie | - |
dc.date.accessioned | 2009-08-31T23:43:46Z | - |
dc.date.available | 2009-08-31T23:43:46Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Biomolecules & Therapeutics 16, 197-202 | en |
dc.identifier.issn | 1225-6110 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8298 | - |
dc.description.abstract | In our previous publication we compared the gene expression profiles on hepatotoxicants exposure to assess the comparability between in vivo and in vitro test systems. We investigated global gene expression from both mouse liver and mouse hepatic cell line treated with thioacetamide (TAA) and identified several common genes. In this study, we selected genes to validate them as potential biomarkers for hepatotoxicity on the relevance of in vitro and in vivo system. Three up-regulated, aquaporin 8 (Aqp8), glutathione peroxidase 1 (Gpx1), succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1) and two down-regulated, DnaJ (Hsp40) homolog subfamily C member 5 (Dnajc5) and tumor protein D52 (Tpd52) genes were tested for their effects in vitro. For characterization of gene function, short interfering RNA (siRNA) for each gene was synthesized and transfected in mouse hepatic cell line, BNL CL.2. Cell viability, mRNA expression level and morphological alterations were investigated. We confirmed siRNA transfection against selected five genes induced down-regulation of respective mRNA expression. siRNA transfection in general decreased cell viability in different degrees and induced morphological changes such as membrane thickening and alterations of intracellular structures. This suggests that these genes could be associated with TAA-induced toxicity. Furthermore, these genes may be used in the investigation of hepatotoxicity for better understanding of its mechanism. | en |
dc.description.sponsorship | This research was supported by a research grant(07141KFDA682) from Korea Food and Drug Administration in 2007. | en |
dc.language.iso | en | - |
dc.publisher | 한국응용약물학회 = The Korean Society of Applied Pharmacology | en |
dc.subject | Thioacetamide | en |
dc.subject | Toxicogenomics | en |
dc.subject | Hepatotoxicity | en |
dc.subject | siRNA | en |
dc.title | Evaluation of Potential Biomarkers for Thioacetamide-induced Hepatotoxicity using siRNA | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 강진석 | - |
dc.contributor.AlternativeAuthor | 염영나 | - |
dc.contributor.AlternativeAuthor | 한을식 | - |
dc.contributor.AlternativeAuthor | 김주환 | - |
dc.contributor.AlternativeAuthor | 이은미 | - |
dc.contributor.AlternativeAuthor | 류덕영 | - |
dc.contributor.AlternativeAuthor | 김영희 | - |
dc.contributor.AlternativeAuthor | 양성희 | - |
dc.contributor.AlternativeAuthor | 김승희 | - |
dc.contributor.AlternativeAuthor | 박순희 | - |
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