S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Preventive and therapeutic effects of gene therapy using silica nanoparticles–binding of GM-CSF gene on white blood cell production in dogs with leukopenia
- Choi, Eun Wha; Koo, Hye Cheong; Shin, Il Seob; Chae, Young Jin; Lee, Jong Hwa; Han, Sei Myoung; Lee, Seung Jun; Bhang, Dong Ha; Park, Yong Ho; Lee, Chang Woo; Youn, Hwa Young
- Issue Date
- Exp Hematol. 2008;36:1091-1097
Our previous study has shown that granulocyte-macrophage colony-stimulating factor (GM-CSF) gene/silica nanoparticles have a leukocytosis effect in normal dogs. Therefore, this study was conducted to determine whether treatment of canine GM-CSF gene/silica nanoparticles has preventive or therapeutic effects in dogs with leukopenia.
Materials and Methods
To induce leukopenia, vinblastine was administered intravenously at a dose of 2 mg/m2 of body surface area on day 0. Then 7.5 μg GM-CSF/nanoparticles (1:100, w/w) were administered intravenously to each of four dogs in the prevention group on day 2 and an equivalent amount of GM-CSF/nanoparticles was administered to the post-nadir group on day 4 (other groups were administered phosphate-buffered saline intravenously).
Therapeutic GM-CSF gene was expressed in peripheral blood mononuclear cells for 10 days and both the prevention and post-nadir groups showed significant increases in white blood cell counts when compared with the control group, as confirmed by complete blood count, differential count, and flow cytometry.
GM-CSF/nanoparticles can be useful for correction of acute leukopenia, such as chemotherapy-induced myelosuppression, without developing neutralizing antibodies.
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